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Method for extracting natural anti-mold agent from alfalfa

A technology of alfalfa grass and antifungal agent, which is applied in the directions of botanical equipment and methods, biocides, fungicides, etc., can solve the problems of high irritation, strong corrosiveness, bad odor, etc., and achieve stable performance, low cost, anti- Mildew effect long lasting effect

Inactive Publication Date: 2018-02-16
袁英良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for extracting natural antifungal agents from alfalfa for traditional chemical antifungal agents, which are highly irritating, corrosive, bad smell, and residual.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In the early stage of harvesting alfalfa, some leaves of different plants of alfalfa were taken, extracted with 45% ethanol under reflux respectively, the extract was centrifuged at 5000r / min, the supernatant was freeze-dried and concentrated to form an extract; Filter paper, punched into 6mm discs, sterilized by dry heat for 1 hour, soaked in the above-mentioned traditional Chinese medicine solution and placed in the refrigerator for 1 hour, then vacuum-dried to obtain filter paper sheets containing medicine, and another 45% ethanol filter paper sheet was used as a blank and 0.5% potassium sorbate filter paper as a positive control; Aspergillus flavus, A. Add equal amount of water to Penicillium and mix evenly to make a mold liquid; pour 10 ml of the mold liquid for testing into Cha's solid culture medium after heating, and pour the mold suspension into the mold suspension after cooling to make an agar plate containing mold in the upper layer ;Paste the drug-containing...

Embodiment 2

[0022] In the early stage of alfalfa harvest, some leaves of different plants of alfalfa were taken. Reversely extract with 45% ethanol, the extract is centrifuged at 5000r / min, the supernatant is freeze-dried and concentrated to form an extract. Take high-quality filter paper with strong water absorption, punch it into 6mm discs, sterilize it by dry heat for 2 hours, soak it in the above-mentioned traditional Chinese medicine solution and put it in the refrigerator for 2 hours, and then vacuum it to dry to get the filter paper containing medicine. 45% ethanol filter paper as blank and 0.5% potassium sorbate filter paper as positive control; Penicillium fungus and Penicillium purpura were mixed with equal amount of water respectively to make mold liquid; 10 ml of the mold liquid for testing was poured into Cha's solid culture medium, and after cooling, the mold suspension was poured into it to make mold liquid. Form an agar plate containing mold in the upper layer; paste the ...

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Abstract

The invention discloses a method for extracting natural anti-mold agent from alfalfa. According to the method, alfalfa is adopted as a raw material, anti-mold tests are implemented to the alfalfa, alfalfa with mold resistance is screened, effective substances of the alfalfa with the mold resistance are extracted, and the natural anti-mold agent is prepared. The natural anti-mold agent prepared byusing the method is high in efficiency in preventing mold in the use process, safe and free of side effects, free of drug resistance, stable in property and long-lasting in anti-mold effect. In addition, the natural anti-mold agent is rich in resource, simple in preparation process and low in cost.

Description

technical field [0001] The invention discloses a method for extracting a natural anti-mold agent from alfalfa, belonging to the field of anti-mold. Background technique [0002] Anti-mildew has always been a problem in the world. Grain and feed are easily polluted by Aspergillus, Penicillium and other molds in high-temperature and high-humidity environments, resulting in mildew. Mildew in grain will cause huge economic losses, and at the same time affect the health of humans and animals. create a threat. If the moldy feed has poor palatability and the nutritional content becomes malignant, it is easy to cause mold poisoning in livestock and poultry, and even induce death. In addition, most molds produce mycotoxins during the reproduction process, such as aflatoxin, brown mycin, etc. These toxins enter the livestock and poultry body, not only causing poisoning of livestock and poultry, but also entering the human body through various channels, thereby endangering human healt...

Claims

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Application Information

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IPC IPC(8): A01N65/20A01P3/00
CPCA01N65/00A01N65/20
Inventor 袁英良唐丹鲁英郭艳芹吴苏日古嘎何建国
Owner 袁英良