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Application of PEDF gene in treating diabetic myocardial damage

A technique for myocardial injury and diabetes, which is applied in the application field of PEDF gene in the treatment of diabetic myocardial injury, and can solve unclear problems

Active Publication Date: 2018-02-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether PEDF is involved in the metabolism of free fatty acids in cardiomyocytes and cardiac lipotoxicity, and whether it is a key molecule in the pathological process of diabetic myocardial injury is still unclear.

Method used

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  • Application of PEDF gene in treating diabetic myocardial damage
  • Application of PEDF gene in treating diabetic myocardial damage
  • Application of PEDF gene in treating diabetic myocardial damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Mouse Body Weight, Blood Glucose and Tissue Weight Determination

[0032] Food and water were not allowed for 12 hours before the end of the experiment. At the end of the experiment, the mice were weighed, and the Johnson & Johnson Wenhao blood glucose meter cut the tail to measure the blood glucose. Intraperitoneal injection of 1% sodium pentobarbital, 80 mg / kg, after the mice were anesthetized, the general photographs were taken. Then the mice were fixed on the dissecting board in the supine position, and the surface was sterilized with 75% disinfectant alcohol. Use surgical scissors to cut open the abdominal cavity of the mouse to the chest cavity, exposing the heart and fat. The right atrial appendage was cut open, and a needle was inserted into the left ventricle to perfuse with PBS until the blood flowed out. The adipose tissue around the heart and epididymis was cut off, rinsed in PBS until no blood stains, and then weighed. Take a picture of the heart. Cut ...

Embodiment 2

[0036] Mouse Cardiac Hypertrophy Assay

[0037] Heart paraffin section HE staining, the main steps are: bake at 60°C for 30 minutes → xylene (Ⅰ) for 10 minutes → xylene (Ⅱ) for 10 minutes → absolute ethanol (Ⅰ) for 3 minutes → absolute ethanol (Ⅱ) for 3 minutes → 95% ethanol (Ⅰ) for 1 minute → 70% ethanol for 1 minute → distilled water for 2 minutes → hematoxylin solution for 5-10 minutes → running water to wash away hematoxylin for 1-3 seconds → 1% hydrochloric acid alcohol for 1-2 seconds → running water for 20 minutes Minutes → overwash with distilled water for 1-2 seconds → 0.5% eosin for 2 minutes → wash with distilled water for 1-2 seconds → 95% ethanol (Ⅱ) for 2-3 seconds → absolute ethanol (Ⅲ) for 3-5 seconds → absolute ethanol (Ⅳ) for 5-10 seconds → xylene (Ⅰ) for 2 minutes → xylene (Ⅱ) for 2 minutes → neutral gum sealing and observation → Image J software to analyze cell size.

[0038] Cardiac hypertrophy is the main pathological feature of diabetic myocardial injur...

Embodiment 3

[0040] Mouse Myocardial Fibrosis Assay

[0041] Masson staining of cardiac paraffin sections, the main steps are: bake at 60°C for 30 minutes → xylene (Ⅰ) for 10 minutes → xylene (Ⅱ) for 10 minutes → absolute ethanol (Ⅰ) for 3 minutes → absolute ethanol (Ⅱ) for 3 minutes → 95% ethanol (I) for 1 minute → 70% ethanol for 1 minute → distilled water for 2 minutes → Weigert's iron hematoxylin staining for 5 minutes → washing with running water for 1-3 seconds → 1% hydrochloric acid alcohol for 1-2 seconds → washing with running water 20 minutes→overwashing with distilled water for 1-2 seconds→dyeing with ponceau magenta solution for 5-10 minutes→quick rinsing with distilled water for a few seconds→treatment with phosphomolybdic acid aqueous solution for about 3-5 minutes→restaining with aniline blue solution for minutes→1% Glacial acetic acid treatment minutes → 95% ethanol (Ⅱ) 2-3 seconds → absolute ethanol (Ⅲ) 3-5 seconds → absolute ethanol (Ⅳ) 5-10 seconds → xylene (Ⅰ) 2 minutes...

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Abstract

The invention discloses application of PEDF gene in treating diabetic myocardial damage. According to the invention, the relationship between the PEDF gene and diabetic myocardial damage is determinedfor the first time, and PEDF gene knockout can lead to a rise in weight and blood sugar, cardiomyocyte hypertrophy, myocardial fibrosis and cardiac dysfunction. It shows that the PEDF gene's functionin diabetic myocardial damage is mainly reflected in the PEDF's effects of improving cardiomyocyte hypertrophy and myocardial fibrosis and improving cardiac function. in allusion to the above functions of the PEDF gene, the PEDF can be used as a medicine for preventing, alleviating or / and treating diabetic myocardial damage; the PEDF can be used as a drug target for screening a medicine for preventing, alleviating or / and treating diabetic myocardial damage; and the PEDF also can be used as a target gene in gene therapy for designing and preparing a medicine for preventing, alleviating or / andtreating diabetic myocardial damage and / or a biological preparation.

Description

technical field [0001] The invention relates to the technical field of function and application research of known genes, in particular to the application of PEDF gene in the treatment of diabetic myocardial injury. Background technique [0002] Cardiovascular complications are the main cause of exacerbation and death of diabetic patients, and about 50-80% of diabetic patients die of cardiovascular disease. Myocardial damage is one of the most important cardiovascular complications of diabetes, and the risk of heart failure in diabetic patients is 2-4 times that of non-diabetic patients. Since American cardiologist Shirley Rubler first proposed the concept of Diabetic cardiomyopathy (DCM) in 1972, the sharp increase in the number of diabetic patients may cause the high incidence of cardiomyopathy, which has attracted scholars and clinicians. of great concern. [0003] Diabetic cardiomyopathy is considered to be the primary myocardial damage that occurs in diabetic patients ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/57A61K45/06A61P3/10A61P9/00C12Q1/68
CPCA61K38/57A61K45/00C12Q1/6883C12Q2600/106
Inventor 蔡卫斌李兴会
Owner SUN YAT SEN UNIV
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