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An ultrasensitive electrochemical immunosensor for multi-residue detection of sulfonamides

A sulfonamide drug and immunosensor technology, which is applied in the fields of food safety testing and analytical chemistry, can solve the problems of complex operation, high testing cost, and difficulty in equipping basic testing departments and units, and achieves simple pretreatment, simple testing operation, and practicality. strong value effect

Inactive Publication Date: 2020-04-24
南京师范大学淮安研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although chromatography occupies the mainstream of sulfonamide drug analysis, its detection cost is high, the operation is complicated, and it requires professional and technical personnel to operate. It is difficult to equip the basic detection departments and units with poor conditions. Still have important practical application value
[0004] Electrochemical sensors have been widely used in the analysis of various harmful substances due to the advantages of simple operation, short detection time, and low cost, but the sulfonamide multi-residue electrochemical immunosensor modified by in situ electrochemical polymerization has not been reported yet.

Method used

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  • An ultrasensitive electrochemical immunosensor for multi-residue detection of sulfonamides
  • An ultrasensitive electrochemical immunosensor for multi-residue detection of sulfonamides
  • An ultrasensitive electrochemical immunosensor for multi-residue detection of sulfonamides

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Preparation of electrochemical immunosensor

[0027] Place the cleaned glassy carbon electrode in a phosphate buffer solution (PBS, 0.067mol / L, pH=8.0) containing 2 μmol / L sulfadiazine, and scan for 10 weeks (scanning speed is 100 mV / S, potential range is -1.0~2.0 V), then place the scanning electrode in a PBS (0.067mol / L, pH=8.0) solution containing 100 μmol / L sulfadiazine and 0.1mol / L NaCl until it is stable (scanning speed is 100 mV / S , The potential range is -1.0~1.0 V), after rinsing and drying, the polysulfadiazine modified PSD-GCE electrochemical immunosensor can be obtained.

Embodiment 2

[0028] Example 2 Detection of Sulfadiazine (SD)

[0029] The PSD-GCE electrochemical immunosensor was prepared in exactly the same way as in Example 1, and it was immersed in a total volume of 50 µL containing 10 μg / mL sulfadiazine antibody and a series of different concentrations of sulfadiazine standard solution, 37 Incubate for 30 minutes at ℃, rinse with phosphate buffer solution and place it in K containing 2 mmol / L 3 [Fe(CN) 6 ] Differential pulse voltammetry (DPV) scan in KCl solution (0.1 mol / L), the DPV curve is as follows figure 1 As shown, the concentration of the curve from top to bottom in the figure is 3000 ng / mL, 1000 ng / mL, 300 ng / mL, 100 ng / mL, 30 ng / mL, 10 ng / mL, 3 ng / mL, 1 ng / mL, 0.3 ng / mL, and 0.1 ng / mL. Record the response current value of the sensor directly in the KCl solution without incubation as I 0 , The response current value measured after incubation with a standard solution containing a certain concentration of sulfadiazine is recorded as I x , The ...

Embodiment 3

[0030] Example 3 Detection of Sulfamethazine (SM2)

[0031] The PSD-GCE electrochemical immunosensor was prepared in exactly the same way as in Example 1, and it was immersed in a total volume of 50 µL containing 10 μg / mL sulfadiazine antibody and a series of different concentrations of sulfadimethazine standard solution Incubate at 37°C for 30 minutes, rinse with phosphate buffer solution, and place it in 2 mmol / L K 3 [Fe(CN) 6 ] Differential pulse voltammetry (DPV) scan in KCl solution (0.1 mol / L), the DPV curve is as follows image 3 As shown, the concentration of the curve from top to bottom in the figure is 400 ng / mL, 100 ng / mL, 25 ng / mL, 15 ng / mL, 5 ng / mL, 2.5 ng / mL, 1 ng / mL, 0.5 ng / mL and 0.2ng / mL. Record the response current value of the sensor directly in the KCl solution without incubation as I 0 , The response current value measured after incubating with a standard solution containing a certain concentration of sulfamethazine is recorded as I x , The response current ...

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Abstract

The invention discloses an application of an electrochemical immunosensor in the aspect of multi-residue detection of sulfonamides. According to the application, firstly, the surface of a glassy carbon electrode is modified with polysulfadiazine with an in-situ electrochemical polymerization method, secondly, detection for four sulfonamides including sulfadiazine, sulfamethazine, sulfacetamide andsulfaguanidine is realized on the basis of cross-immune response of sulfadiazine antibodies and sulfonamides, wherein the limit of detection is in a range of 0.06-0.15 ng / mL, and linear range is 0.1-1500 ng / mL or 0.3-500 ng / mL.

Description

Technical field [0001] The invention belongs to the technical field of food safety detection and analytical chemistry, and specifically relates to the application of an electrochemical immunosensor modified by an in-situ electrochemical polymerization method in the multi-residue detection of sulfa drugs. Background technique [0002] Sulfonamides are the general term for a class of drugs containing the p-aminobenzenesulfonamide structure. They have been used as antibiotics for more than 70 years due to their broad antibacterial spectrum, low price, and stable properties. Sulfonamides have side effects such as allergies, inhibition of enzyme activity in the body, and intestinal flora imbalance. If humans take them in large amounts, they often have great risks. my country, Europe and the United States and other countries have stipulated that the maximum residue limit (MRL) of total sulfonamides in animal foods is 0.1 mg / kg. The current analytical techniques for sulfa drugs mainly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N27/48
CPCG01N27/48G01N33/543
Inventor 赵波王雅娟丁利霞邵科峰
Owner 南京师范大学淮安研究院
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