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Method for co-producing algal polysaccharide and algal protein from alga

A technology of seaweed protein and seaweed polysaccharide, which is applied to the preparation methods of peptides, chemical instruments and methods, organic chemistry and other directions, can solve the problems of high cost, strict operating conditions, and complexity, and achieves improved utilization, simple operation and large output. Effect

Active Publication Date: 2018-02-23
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN 104403018A discloses a method for extracting seaweed polysaccharides under high pressure, and patent CN 04892785A discloses a method for extracting seaweed polysaccharides under ultra-high pressure. Both methods are carried out under high pressure, which requires high equipment and high cost Patent CN105580975A discloses a method for extracting seaweed protein by microbial fermentation, which requires microbial cultivation to extract protein from seaweed, and the operating conditions are strict and complicated; patent CN104341536A discloses a method for extracting nutrients from seaweed , the method uses compound enzymes to pretreat seaweed to extract sodium alginate, and the cost is relatively high; patent CN103951737A discloses a method for extracting glycoproteins from seaweeds, which only extracts glycoproteins from seaweeds; patent CN103739726A publishes A method for extracting polysaccharides from seaweed was developed. The polysaccharides were extracted under normal pressure, protein was removed by sevage method, and polysaccharides were purified by running water dialysis. Although the method was simple to operate, the small molecule impurities in polysaccharides could not be completely removed by running water dialysis and the sevage reagent Strong toxicity
The published method only involves a method of extracting seaweed polysaccharide or seaweed protein, and high-purity seaweed polysaccharide and seaweed protein cannot be obtained at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for co-producing seaweed polysaccharides and seaweed protein is carried out according to the following steps:

[0042] (1) Wash the Enteromorpha to remove impurities, dry and pulverize, and cross an 80-mesh sieve;

[0043] (2) Weigh 15g of Enteromorpha powder, add 75mL of 95(v / v)% ethanol, reflux for 2.5h, filter, add 300mL extractant for microwave extraction 3 times, microwave power is 1700W, interval 5min, each time 5min, extraction temperature to 80°C, filter, combine the filtrates and concentrate to 100 mL, add solid ammonium sulfate to saturation, let stand for 36 hours, filter, and save the filtrate for future use. The filtered solid was dissolved in water, dialyzed under running water for 2 days, the dialysate was concentrated to 1 / 4 of the original volume at 80°C, and freeze-dried to obtain Enteromorpha protein 2.12g with a purity of 93%;

[0044] The extractant is dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution, which is ...

Embodiment 2

[0047] A method for co-producing seaweed polysaccharides and seaweed protein is carried out according to the following steps:

[0048] (1) Ulva is cleaned of impurities, dried and crushed, and passed through an 80-mesh sieve;

[0049] (2) Weigh 15g of Ulva powder, add 75mL of 95% ethanol, reflux for 2.5h, filter, add 300mL extractant for microwave extraction 3 times, microwave frequency is 1700W, interval 4min, each 8min extraction temperature is 80°C, combine filtrate And concentrate to 100mL, add solid ammonium sulfate to saturation, let stand for 36h, filter, and save the filtrate for future use. The filtered solid was dissolved, dialyzed under running water for 2 days, the dialysate was concentrated to 1 / 4 of the original volume at 80°C, and freeze-dried to obtain 2.00 g of seaweed protein with a purity of 92.3%;

[0050] The extractant is dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution, which is prepared from dipotassium hydrogen phosphate a...

Embodiment 3

[0053] A method for co-producing seaweed polysaccharides and seaweed protein is carried out according to the following steps:

[0054] (1) Wash Undaria pinnatae to remove impurities, dry and pulverize, and pass through an 80-mesh sieve;

[0055] (2) Weigh 15g undaria pinnatifida powder, add 75mL95% ethanol, reflux for 2.5h, filter, add 300mL extractant microwave extraction 3 times, microwave frequency is 1700W, interval 3min, each 6min, extraction temperature is 80 ℃, combine The filtrate was concentrated to 100 mL, solid ammonium sulfate was added to make it saturated, allowed to stand for 36 hours, filtered, and the filtrate was stored for future use. The filtered solid was dissolved, dialyzed under running water for 2 days, and the dialysate was concentrated to 1 / 4 of the original volume at 80°C and freeze-dried to obtain 1.28 g of seaweed protein with a purity of 92%;

[0056] The extractant is dipotassium hydrogen phosphate-potassium dihydrogen phosphate buffer solution,...

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PUM

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Abstract

The invention discloses a method for co-producing algal polysaccharide and algal protein from alga. The method comprises the following steps of 1, preprocessing raw materials; 2, extracting and purifying the algal protein; 3, extracting and purifying the algal polysaccharide. The obtained algal polysaccharide and algal protein are directly extracted from the alga in one step, and therefore the utilization rate of the alga is dramatically improved.

Description

technical field [0001] The invention belongs to the technical field of comprehensive utilization of seaweed, and in particular relates to a method for co-producing seaweed polysaccharide and seaweed protein from seaweed. Background technique [0002] Seaweed is rich in reserves and contains a variety of active ingredients, which have important applications in food, medicine, daily chemicals, fertilizers, feed and other fields. Therefore, the comprehensive utilization of seaweed has attracted more and more attention. Seaweed mainly contains lipids, proteins, polysaccharides and other active ingredients. If the active ingredients in seaweed can be separated and purified, the utilization rate of seaweed can be greatly improved. Among the ingredients contained in seaweed, seaweed polysaccharide and seaweed protein have the highest content, and their application is also the most extensive. The main sources of seaweed polysaccharides can be divided into three categories: fucoida...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07K1/14C07K1/16C07K1/30C07K1/36
CPCC07K1/145C07K1/16C07K1/30C07K1/303C07K1/36C08B37/0003
Inventor 李金花宿斌王宗花
Owner QINGDAO UNIV
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