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Method for establishing memory impairment animal model of environmental pollutants

A technology for environmental pollutants and animal models, which is applied in the field of establishing animal models of memory impairment caused by environmental pollutants, can solve problems such as affecting Alzheimer's disease case studies, unable to fully simulate diffuse brain lesions, etc., and shorten the feeding time. Time limit, accelerated aging, cost saving effect

Inactive Publication Date: 2018-02-27
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a method for establishing an animal model of memory impairment caused by environmental pollutants, which solves the problem that simple injection of Aβ cannot completely simulate diffuse brain lesions and affects case studies of Alzheimer's disease

Method used

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  • Method for establishing memory impairment animal model of environmental pollutants

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Embodiment 1

[0025] A method for establishing an animal model of memory impairment caused by environmental pollutants. The establishment of the animal model takes 5 weeks. The first 3 weeks are the low-dose administration period, and the fourth week is the high-dose administration period;

[0026] Among them, the low-dose administration period takes 7 days as a cycle operation cycle, and the cycle is 3 times, and the operation steps of each cycle operation cycle are as follows:

[0027] On the 1st-6th day, the manganese sulfate solution of concentration 2mg / ml is given intraperitoneally to animal every day, and injection volume is 1mL / kg body weight; 1 μL / kg body weight, and 2 hours after the injection of β-amyloid protein solution, the animals were exposed to a low-pressure oxygen environment of 60±2KPa for 10 minutes;

[0028] Among them, the high-dose administration period is 7 days in total, and the specific operation is as follows:

[0029] On the 1st-6th day, the manganese sulfate s...

Embodiment 2

[0034] A method for establishing an animal model of memory impairment caused by environmental pollutants. The establishment of the animal model takes 5 weeks. The first 3 weeks are the low-dose administration period, and the fourth week is the high-dose administration period;

[0035] Among them, the low-dose administration period takes 7 days as a cycle operation cycle, and the cycle is 3 times, and the operation steps of each cycle operation cycle are as follows:

[0036] On the 1st to 6th days, the manganese sulfate solution with a concentration of 2mg / ml was injected intraperitoneally to the animal every day, and the injection volume was 1.5mL / kg body weight; 1 μL / kg body weight, and 2 hours after the injection of β-amyloid protein solution, the animals were exposed to a low-pressure oxygen environment of 60±2KPa for 20 minutes;

[0037] Among them, the high-dose administration period is 7 days in total, and the specific operation is as follows:

[0038] On days 1-6, intr...

Embodiment 3

[0043] A method for establishing an animal model of memory impairment caused by environmental pollutants. The establishment of the animal model takes 5 weeks. The first 3 weeks are the low-dose administration period, and the fourth week is the high-dose administration period;

[0044] Among them, the low-dose administration period takes 7 days as a cycle operation cycle, and the cycle is 3 times, and the operation steps of each cycle operation cycle are as follows:

[0045]On the 1st-6th day, the manganese sulfate solution of concentration 2mg / ml is given intraperitoneally to animal every day, and injection volume is 2mL / kg body weight; 1 μL / kg body weight, and 2 hours after the injection of β-amyloid protein solution, the animals were exposed to a low-pressure oxygen environment of 60±2KPa for 10 minutes;

[0046] Among them, the high-dose administration period is 7 days in total, and the specific operation is as follows:

[0047] On the 1st-6th day, the manganese sulfate so...

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Abstract

The invention belongs to the technical field of biology and discloses a method for establishing a memory impairment animal model by environmental pollutants. Five weeks are consumed by an animal modelestablishment process, the previous three weeks serve as a low-dose administration period, the fourth week serves as a high-dose administration period, and the fifth week serves as a natural growth period, wherein a cyclic operation period includes seven days in the low-dose administration period, and three cycles are performed; the high-dose administration period has a duration of seven days; and water is freely fed during the animal model establishment period. According to the method, a low pressure oxygen environment, a manganese sulfate solution and a beta-amyloid protein solution complexfactors are taken as environmental pollutants, a progressive administration manner is adopted, lesions of a rat brain memory neuron area (a hippocampal area) are gradually induced by virtue of accumulation effects of the environment and drugs, thereby establishing a stable memory impairment animal model. The brain lesions of rats are in diffuse distribution, the reproducibility is excellent, anddiffuse brain lesions of Alzheimer disease can be well simulated.

Description

technical field [0001] The invention belongs to the technical field of biology, and in particular relates to a method for establishing an animal model of memory damage caused by environmental pollutants. Background technique [0002] Alzheimer's disease (AD) is a degenerative disease of the central nervous system that often occurs in middle-aged and elderly people. At present, there is no effective cure. Studies have found that the early lesions of Alzheimer's disease are mainly related to the decrease in the function of the cholinergic system in the brain, including senile plaques formed by the aggregation of β-amyloid protein in the brain, and neurofibrillary tangles formed by the abnormal aggregation of tau protein in neurons. , different degrees of neuronal apoptosis or loss, and astroglial hyperplasia and hypertrophy. The clinical symptoms of patients are mainly characterized by progressive memory loss and cognitive dysfunction. [0003] In order to further study the p...

Claims

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Application Information

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IPC IPC(8): A61K33/32A61K38/17A01K67/02
CPCA01K67/02A01K2227/105A01K2267/03A61K33/32A61K38/1709
Inventor 田建英王晨王天琪王忧美
Owner NINGXIA MEDICAL UNIV
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