A method for establishing an animal model of memory impairment caused by environmental pollutants
A technology for environmental pollutants and animal models, which is applied in the field of establishing animal models of memory impairment caused by environmental pollutants, can solve problems such as the inability to fully simulate diffuse brain lesions and affect the case study of Alzheimer's disease, and shorten the feeding time. Time limit, accelerated aging, cost saving effect
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Embodiment 1
[0025] A method for establishing an animal model of memory impairment caused by environmental pollutants. The establishment of the animal model takes 5 weeks. The first 3 weeks are the low-dose administration period, and the fourth week is the high-dose administration period;
[0026] Among them, the low-dose administration period takes 7 days as a cycle operation cycle, and the cycle is 3 times, and the operation steps of each cycle operation cycle are as follows:
[0027] On the 1st-6th day, the manganese sulfate solution of concentration 2mg / ml is given intraperitoneally to animal every day, and injection volume is 1mL / kg body weight; 1 μL / kg body weight, and 2 hours after the injection of β-amyloid protein solution, the animals were exposed to a low-pressure oxygen environment of 60±2KPa for 10 minutes;
[0028] Among them, the high-dose administration period is 7 days in total, and the specific operation is as follows:
Embodiment 2
[0034] A method for establishing an animal model of memory impairment caused by environmental pollutants. The establishment of the animal model takes 5 weeks. The first 3 weeks are the low-dose administration period, and the fourth week is the high-dose administration period;
[0035] Among them, the low-dose administration period takes 7 days as a cycle operation cycle, and the cycle is 3 times, and the operation steps of each cycle operation cycle are as follows:
[0036] On days 1-6, intraperitoneally inject manganese sulfate solution with a concentration of 2mg / ml into the animal, and the injection volume is 1.5mL / kg body weight; 1 μL / kg body weight, and 2 hours after the injection of β-amyloid protein solution, the animals were exposed to a low-pressure oxygen environment of 60±2KPa for 20 minutes;
[0037] Among them, the high-dose administration period is 7 days in total, and the specific operation is as follows:
[0038] On days 1-6, intraperitoneally inject manganese...
Embodiment 3
[0043] A method for establishing an animal model of memory impairment caused by environmental pollutants. The establishment of the animal model takes 5 weeks. The first 3 weeks are the low-dose administration period, and the fourth week is the high-dose administration period;
[0044] Among them, the low-dose administration period takes 7 days as a cycle operation cycle, and the cycle is 3 times, and the operation steps of each cycle operation cycle are as follows:
[0045]On the 1st-6th day, the manganese sulfate solution of concentration 2mg / ml is given intraperitoneally to animal every day, and injection volume is 2mL / kg body weight; 1 μL / kg body weight, and 2 hours after the injection of β-amyloid protein solution, the animals were exposed to a low-pressure oxygen environment of 60±2KPa for 10 minutes;
[0046] Among them, the high-dose administration period is 7 days in total, and the specific operation is as follows:
[0047] On the 1st-6th day, the manganese sulfate so...
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