A functional soybean whey drink with refreshing flavor and production method thereof
A soybean whey and production method technology, applied in the direction of lactobacillus, milk substitutes, dairy products, etc., can solve the problem of beany smell, achieve the regulation of intestinal flora, benefit cardiovascular health, and increase the antioxidant activity index high effect
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Embodiment 1
[0050] Example 1 Isolation, identification and fermented soybean whey characteristics of Lactobacillus rhamnosus
[0051] (1) Lactobacillus rhamnosus (Lactobacillus rhamnosus) is isolated from Physalis water in Kaiping, Guangdong. The separation and purification method is as follows: Take 1ml of Physalis water supernatant, dilute and spread it on MRS agar medium, and culture it upside down at 37°C overnight , pick a single colony on the plate to obtain strains.
[0052] (2) Genomic DNA extracted by a DNA extraction kit was subjected to agarose gel electrophoresis, and then 16S rDNA primers were designed for PCR amplification. The purified and recovered 16S rDNA fragments were sequenced, and the sequencing results are shown in the sequence table SEQ.ID.NO1. Then use BLAST software to compare with the reported 16Sr DNA, and the comparison results show that the similarity between the DNA sequence and Lactobacillus rhamnosus reaches 99%, so it is identified as Lactobacillus rhamn...
Embodiment 2
[0055] Embodiment 2 (comparative example) unfermented soybean whey drink
[0056] (1) Take 2×300mL of fresh tofu yellow slurry water, add 15g of sucrose, fully dissolve, filter to remove insoluble matter, treat with high-pressure steam at 121°C for 15min, cool down and refrigerate overnight at 4°C. Take 300ml subpackages, after chemical analysis and sensory evaluation, it is found that: ① The acidity value of soybean whey beverage is 16.49°T, the overall acceptability score of sensory evaluation is 5.63 points, of which the odor score is 5.25 points, and the color score is 8.25 points , with a taste score of 5.75. ② The ability of fermented yellow pulp water to scavenge DPPH free radicals is 13.81 μmol Trolox / L, and the ability to reduce Fe 3+ The capacity is 436μmol Trolox / L.
[0057] (2) Juice the fresh pineapple pulp, filter to remove the filter residue, treat with high-pressure steam at 115°C for 15 minutes, and cool for later use.
[0058] (3) Take 15 mL of the pineapp...
Embodiment 3
[0060] Example 3 Streptococcus thermophilus (Streptococcus.thermophilus) joint Lactobacillus amylolyticus (Lactobacillus amylolyticus) fermented soybean whey drink
[0061] (1) Take 300mL of fresh tofu yellow slurry water, add 15g of sucrose, fully dissolve, filter to remove insoluble matter, treat it with high-pressure steam at 121°C for 15min, and cool it for later use.
[0062] (2) Take 3mL Streptococcus thermophilus seed culture solution and 3mL Lactobacillus amyloliquefaciens seed culture solution and inoculate them into the yellow slurry water prepared in (1) above, shake well, divide into 50mL small beakers, and ferment at 42°C 36h. The preservation number of Streptococcus.thermophilus is CGMCC NO.11943; it was preserved on December 28, 2015, and its location is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.
[0063] (3) Centrifuge the soybean whey beverage after the above-mentioned fermentation, and take the supernatant and store it in a refrigerator at...
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