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Cross-linked starch biosynthesis recombinant gene CBII and application thereof

A biosynthesis and recombinant gene technology, applied in the field of biomedicine, can solve problems such as unenhanced starch accumulation, and achieve the effects of improving vitality, increasing starch accumulation, and increasing starch viscosity

Inactive Publication Date: 2018-03-06
NINGXIA UNIVERSITY
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Problems solved by technology

[0020] GBSSI antisense RNA inhibited the expression of GBSSI, obtained reduced amylose synthesis or only amylopectin synthesis, but did not enhance starch biosynthesis and increase starch accumulation

Method used

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  • Cross-linked starch biosynthesis recombinant gene CBII and application thereof

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Embodiment Construction

[0029] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0030] In the present invention, glgC55 (GenBank: AY943834), the gene encoding adenosine diphosphate glucose pyrophosphorylase, was isolated from Escherichia coli JM109-3 mutant strain, and the starch synthase encoding gene GBSSIcDNA combined with starch granules was isolated from potato ( DQ387451) and its promoter DNA.

[0031] glgC55 encodes an AGPase subunit (AAY18580) consisting of 431 amino acids, in which there are 4 specific mutations, they are K39E, V71A, I248V and K304E, the former two appear in the Rossmann fold region of the N-terminal metabolic group of the oligomeric subunit The latter two occur within the C-terminal heterogeneously regulated and oligomerized LbH groups of the oligomeric subunits.

[0032]The present invention uses glgC55, GBSSIcDNA and its promoter DNA to construct the GBSSI:g...

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Abstract

The invention discloses a cross-linked starch biosynthesis recombinant gene CBII and application thereof. The nucleotide sequence is shown as SEQ ID NO:1, and the recombinant gene which not only can inhibit the synthesis of amylose but also can increase starch content is obtained. The invention which provides the cross-linked starch biosynthesis recombinant gene CBII and the application thereof separates out glgC55 of the ADP-glucose pyrophosphorylase coding gene from a mutant strain of escherichia coli JM109-3, and separates out a starch grain-combined starch synthase coding gene GBSSIcDNA and a promoter DNA thereof from potatoes.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and discloses a cross-linked starch biosynthesis recombinant gene CBII and its application. Background technique [0002] Starch is one of the most common and cheapest photopolymerizable carriers next to cellulose. Starch has a single composition, is easy to store, and has a wide range of uses. It is an important industrial raw material for building materials, textiles, papermaking, and oil extraction. In particular, the chemical energy stored in it is easy to convert and utilize again. For this reason, a new understanding of starch at a strategic level is in the ascendant. [0003] Starch comes from plant seeds (such as rice, wheat, corn, etc.), tubers (such as potatoes, etc.), etc. Due to food composition and starch characteristics, potato starch is a widely accepted and used industrial raw material; the starch yield of potato production areas in my country (The amount of tubers required to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/82A01H5/00A01H6/82
Inventor 姚新灵
Owner NINGXIA UNIVERSITY
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