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SSR Molecular Markers of Seedless Major Qtl Loci in Grape Fruit

A molecular marker, grape technology, applied in the direction of determination/inspection of microorganisms, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of low breeding rate of offspring, long breeding cycle, hindering the breeding process of seedless grapes, etc.

Active Publication Date: 2020-10-23
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional cross-breeding cycle is long and the offspring selection rate is low, which greatly hinders the process of seedless grape breeding

Method used

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  • SSR Molecular Markers of Seedless Major Qtl Loci in Grape Fruit
  • SSR Molecular Markers of Seedless Major Qtl Loci in Grape Fruit
  • SSR Molecular Markers of Seedless Major Qtl Loci in Grape Fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Acquisition of SSR Molecular Markers of Seedless Major QTL Loci in Grape Fruit

[0024] (1) The female parent 'Red Earth' was crossed with the male parent 'Moritani Seedless' to obtain 131 F1 populations;

[0025] (2) RAD-seq (restriction site-associated DNA sequencing) was performed on the two parents and 131 F1 populations; firstly, the genomic DNA of each individual of the two parents and 131 F1 populations was extracted, and the quality was tested Afterwards, carry out enzyme digestion, electrophoresis to recover DNA fragments of the required length, add adapters for cluster preparation, and perform sequencing on the machine; then use SOAP comparison software to compare the sequencing sequence to the reference genome sequence (NCBI-GCF-000003745.3-12X) ; According to the SOAP comparison results, use SAMtools software to generate pileup and glf files required by CaSFS software, use CaSFS software to identify the situation of each site in the population, and...

Embodiment 2

[0030] Early detection of the seedless character of embodiment 2 grape fruit

[0031] At the seedling stage, the whole genome DNA of the 'female' Red Earth', the paternal 'Moritani Seedless' and the hybrid F1 population of the two parents were extracted as templates, and the primer pair of molecular marker VvSD10 was used as the amplification primer, and HRM (high resolution Melting curve analysis) technology for detection and analysis; compared with the melting curves of the female parent 'Red Earth' and the male parent 'Moritani Seedless', if the color of the melting curve of the offspring is consistent with that of the female parent, the offspring will be predicted The sample fruit has a seed. If the melting curve of the offspring is consistent with the color of the male parent, it is predicted that the fruit of the offspring sample is seedless; the results show that in the 131 F1 populations, 101 offspring individuals have the same melting curve color as the male parent. ,...

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Abstract

The invention belongs to the field of molecular breeding of vitis vinifera and in particular provides an SSR molecular marker for seedless main-effect QTL (Quantitative Trait Loci) sites of vitis vinifera fruits. The seedless main-effect QTL sites of the vitis vinifera fruits are detected in the 18th linkage group of a genetic linkage map constructed by a female parent 'Red Globe' and a male parent 'Centennial Seedless', wherein the site contribution rate is 77.9%. The SSR molecular marker VvSD10 chained with the interval is screened in the interval in which the seedless main-effect QTL sitesof the vitis vinifera fruits are located and can be used for early detection of seedless traits of the vitis vinifera fruits.

Description

technical field [0001] The invention belongs to the field of grape molecular breeding, and specifically provides an SSR molecular marker of a seedless main effect QTL site of grape fruit. Background technique [0002] Grape( Vitis vinifera L.) belongs to the berry of the genus Vitis vinifera, which is the second largest cultivated fruit tree in my country and can be widely used in wine making, fresh food, juice making, and drying. Among them, seedless grapes occupy the first place. Seedless grape juice is rich in juice, high in sugar content, convenient to eat, and of good quality, and is deeply loved by consumers. In developed countries, more than 50% of table and dried grapes consumed are seedless grapes. In my country, Xinjiang is the main grape producing area, and most of them are seedless grape varieties. Therefore, seedless grape breeding has become one of the main goals of grape breeding. [0003] In the breeding of seedless grape varieties, the main way is conv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张颖刘崇怀马亚茹樊秀彩姜建福
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI