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Avian Influenza Virus Infection or Avian Influenza Vaccine Immunotyping Kit

A bird flu virus and detection kit technology, applied in the direction of virus peptides, peptide sources, etc., can solve the problems of chickens dying in batches, affecting egg production of laying hens, and high lethality

Active Publication Date: 2020-03-27
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, for chickens, the H5 subtype is highly pathogenic, which will lead to the death of chickens in batches; the H7 subtype has a high lethality rate, and the infection has been increasing in recent years; although the H9 subtype is low pathogenicity, But it will significantly affect the egg production of laying hens

Method used

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  • Avian Influenza Virus Infection or Avian Influenza Vaccine Immunotyping Kit
  • Avian Influenza Virus Infection or Avian Influenza Vaccine Immunotyping Kit
  • Avian Influenza Virus Infection or Avian Influenza Vaccine Immunotyping Kit

Examples

Experimental program
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Effect test

Embodiment

[0093] 1. Preparation and confirmation of peptides

[0094] The polypeptides used in the examples were synthesized by Gil Biochemical (Shanghai) Co., Ltd. and confirmed by mass spectrometry. among them,

[0095] SEQ ID NO:1: LMENERTLDFHDSNVKNLYD.

[0096] SEQ ID NO: 2: QQFELIDNEFNEVEKQIGNV.

[0097] SEQ ID NO: 3: ADSEMDKLYERVKRQLRENA.

[0098] SEQ ID NO: 4: FHKCDDDCMASIRNNTYDHR.

[0099] 2. Preparation of the kit (detection chip)

[0100] Use conventional methods to spot the polypeptide solutions shown in SEQ ID NO: 1 to 4 on a conventional solid phase carrier for ELISA, and spot a chicken IgY spot as a positive quality control spot and a PB spot as a negative control spot. Point, prepared a detection chip.

[0101] 3. Test with the kit

[0102] Inspection steps

[0103] (1) Before starting the test, add purified water or distilled water to dilute the concentrated cleaning solution in a ratio of 1:10, and use it directly after the dilution is completed. Use a pipette to add 2 mL of cl...

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Abstract

The invention relates to a bird flu virus infection or bird flu vaccine immune typing kit. The detection kit of the present invention includes one or more solid supports, and a specific polypeptide set independently linked to the one or more solid supports.

Description

Technical field [0001] The invention mainly relates to a kit for poultry and a diagnostic method. Specifically, the present invention relates to kits and diagnostic methods that can be used for typing avian influenza virus and / or avian influenza vaccine immunity. Background technique [0002] Avian influenza is a disease syndrome of poultry and wild birds caused by influenza A virus. The disease was first discovered in Italy in 1878 and was called "chicken plague" at the time. It was not confirmed as an infection with the H7 strain of influenza A virus until 1955. [0003] In recent years, chicken and bird flu have been on the rise in all countries in the world. In particular, the bird flu outbreak in Hong Kong since 1997 has posed a threat to human health. Avian influenza is a kind of infectious disease listed by the International Veterinary Administration (OIE) and the Ministry of Agriculture, which poses a huge threat to the chicken industry. It can be said that the effective...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/11
Inventor 马宏伟金燕萍李彤王晓莹
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI