Preparation of sugarcane leaf polysaccharide and its application to treat and prevent diabetes
A sugarcane leaf, diabetes technology, applied in the field of diabetes treatment, can solve problems such as not being able to truly simulate diabetes
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[0015] Preventive effect and mechanism of sugarcane leaf polysaccharide on diabetes in NOD mice
[0016] 1 Experimental instruments and materials
[0017] 1.1 Experimental Instruments
[0018] High-speed refrigerated centrifuge, model: X1R, Thermo Scientific, USA; -20°C refrigerator, model: BC-45M, Guangdong Midea Group Co., Ltd.; CO 2 Constant temperature incubator, model: MCO-18AIC, Japan SANYO company; full-wavelength microplate reader, model: Epoch, American BioTek Co., Ltd.; electronic scale, model: JCS-300, Zhejiang Kaifeng Group Co., Ltd.; Roche excellent blood glucose meter And blood glucose test strips, Germany Roche Co., Ltd.
[0019] 1.2 Experimental reagents
[0020] FTY720, TCI (Shanghai) Chemical Industry Development Co., Ltd., lot number F1018; mouse insulin Elisa kit, lot number 15019657, mouse IL-2 Elisa kit, lot number 01019659, mouse TNF-alpha Elisa kit, lot number 03019658, Mouse IL-17 Elisa kit, batch number 03019660, Wuhan Huamei Technology Co., Ltd.;...
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