Response-surface-method based enzyme extraction method and content determination of Chinese violet apigenin

A viola chinensis, response surface method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Inactive Publication Date: 2018-03-09
TONGREN POLYTECHNIC COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymatic extraction has the advantages of mild extraction conditions, low energy consumption, and no special requirements for equipment, so that enzymatic extraction of flavono

Method used

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  • Response-surface-method based enzyme extraction method and content determination of Chinese violet apigenin
  • Response-surface-method based enzyme extraction method and content determination of Chinese violet apigenin
  • Response-surface-method based enzyme extraction method and content determination of Chinese violet apigenin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] (1) drying and pulverizing Viola Viola into 80 meshes to obtain Viola Viola powder;

[0079] Weigh 10.00 g of viola chinensis powder, add acetic acid-sodium acetate buffer solution, adjust the pH to 4.6, add 1.8% cellulase according to the mass ratio of medicinal materials, and enzymatically hydrolyze at 50°C for 155 minutes;

[0080] Concentrate the crude extract to a thick paste with a rotary evaporator, and dry it in a vacuum oven for 24 hours to obtain the crude extract.

[0081] Weigh the above-mentioned crude matter, dissolve it with anhydrous methanol, and after dilution, use high-performance liquid chromatography to measure violadin apigenin, and according to the apigenin yield Y=[(apigenin content×extract weight) / zihuadi D medicinal material weight] × 100%. The calculated yield of apigenin was 41.26μg / g.

Embodiment 2

[0083] Drying and pulverizing Viola Viola into 90 meshes to obtain Viola Viola powder;

[0084] Weigh 10.00 g of viola powder, add acetic acid-sodium acetate buffer solution, adjust the pH to 5.5, add 2.4% cellulase according to the mass ratio of medicinal materials, and enzymolyze at 60°C for 210 minutes;

[0085] Concentrate the crude extract to a thick paste with a rotary evaporator, and dry it in a vacuum oven for 30 hours to obtain the crude extract.

[0086] Weigh the above-mentioned crude matter, dissolve it with anhydrous methanol, and after dilution, use high-performance liquid chromatography to measure violadin apigenin, and according to the apigenin yield Y=[(apigenin content×extract weight) / zihuadi D medicinal material weight] × 100%. The calculated yield of apigenin was 41.05μg / g.

Embodiment 3

[0088] Drying and pulverizing Viola Viola into 70 meshes to obtain Viola Viola powder;

[0089] Weigh 10.00 g of viola chinensis powder, add acetic acid-sodium acetate buffer solution, adjust the pH to 3.5, add 1.2% cellulase according to the mass ratio of medicinal materials, and enzymatically hydrolyze at 40°C for 90 minutes;

[0090] Concentrate the crude extract to a thick paste with a rotary evaporator, and dry it in a vacuum oven for 12 hours to obtain the crude extract.

[0091] Weigh the above-mentioned crude matter, dissolve it with anhydrous methanol, and after dilution, use high-performance liquid chromatography to measure violadin apigenin, and according to the apigenin yield Y=[(apigenin content×extract weight) / zihuadi D medicinal material weight] × 100%. The calculated yield of apigenin was 40.24μg / g.

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Abstract

The invention relates to a response-surface-method based enzyme extraction method and content determination of Chinese violet apigenin, and belongs to the technical field of traditional Chinese medicine. The response-surface-method based enzyme extraction method and the content determination have the advantages that the Chinese violet apigenin is extracted through cellulose enzyme extraction, optimal process parameters are acquired through response surface analysis based screening of enzymolysis temperature, enzymolysis time, enzymolysis pH value and cellulose consumption in the process of cellulose enzyme extraction, and the yield of the Chinese violet apigenin extracted according to the process parameters reaches more than 38.50microgram/gram; the process is eco-environment friendly, stable and feasible, residual solvents and solvent pollution both generated during extraction by organic solvents are avoided effectively, and safety and environment protection can be achieved while theChinese violet apigenin is extracted efficiently.

Description

technical field [0001] The invention relates to the technical field of extracting apigenin from Viola chinensis, in particular to an enzymatic extraction method based on response surface method. Background technique [0002] Viola philippicassp munda W.Becker is a viola plant of the Viola family, a perennial herb that can be harvested in spring, summer, and autumn. It has the effects of clearing away heat and detoxification, clearing away heat and promoting dampness, and is widely distributed as a folk Chinese herbal medicine. Apigenin is the main component of its flavonoids. Flavonoids have anti-oxidation, anti-cancer, prevent cardiovascular disease, anti-inflammatory, anti-allergic, analgesic, antibacterial, antiviral and other effects. [0003] In the prior art, Chinese patent CN200910170069.8 discloses a method for extracting apigenin from Viola dichotoma, using methanol ultrasonic treatment, centrifugation, concentration, extraction, macroporous adsorption resin LSA-10 ...

Claims

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Application Information

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IPC IPC(8): C12P17/06G01N30/02
CPCC12P17/06G01N30/02
Inventor 韩建军宁娜
Owner TONGREN POLYTECHNIC COLLEGE
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