Method for improving protein solubility of rapeseed cakes
A rapeseed cake and protein technology, applied in animal feed, animal feed, application, etc., can solve the problems of low protein solubility and difficulty in being fully utilized by animals, so as to achieve high protein solubility, realize value-added utilization, and facilitate absorption and utilization. Effect
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[0014] According to a preferred embodiment of the present invention, in the rapeseed cake raw material, based on the total weight of the rapeseed cake raw material, the content of protein in the rapeseed cake raw material is 33-38% by weight, and the content of phytic acid is 0 -3% by weight, the content of fat is 6-15% by weight, the content of ash is 3-10% by weight, and the content of water is 0-8% by weight. The method provided by the invention effectively improves the protein solubility of the rapeseed cake without changing the content of the above components in the raw material of the rapeseed cake. The rapeseed cake raw material also contains fibers, inorganic salts, metals and other unavoidable impurities. The various components contained in the rapeseed cake raw material satisfy the sum of 100% by weight.
[0015] According to a preferred embodiment of the present invention, the particle size of the rapeseed cake raw material is not greater than 0.38mm, preferably no...
Embodiment 1
[0039] (1) Get 20g of rapeseed cake raw material (moisture content 5% by weight, particle diameter is not more than 0.25mm), pre-weighed water 14.54g, sodium hydroxide 0.497g and alkaline protease 9500U; Sodium hydroxide and alkaline protease Dissolve in pre-weighed water to form a solution, add the sodium hydroxide solution to the rapeseed cake raw material and stir evenly with a small spoon, then add the enzyme solution and stir evenly, so that the water content of the final sample is 45%, and the pH is 8.0 ±0.2;
[0040] (2) Put the treated samples in a bag and seal them, and enzymatically hydrolyze them in an incubator at 55±1°C for 48 hours, and then dry them in an oven at 60°C for 4 hours to obtain rapeseed cake S1. The measurement results of the protein solubility of the obtained rapeseed cake S1 are listed in Table 1.
Embodiment 2
[0042] (1) Get 20g of rapeseed cake raw material (moisture content 5% by weight, particle diameter is not more than 0.25mm), pre-weighed water 22.22g, sodium hydroxide 0.676g and alkaline protease 19000U; Sodium hydroxide and alkaline protease Dissolve in pre-weighed water to form a solution, add the sodium hydroxide solution to the rapeseed cake raw material and stir evenly with a spoon, then add the enzyme solution and stir evenly, so that the water content of the final sample is 55%, and the pH is 9.0 ±0.2;
[0043] (2) Put the treated sample in a bag and seal it, and enzymolyze it in an incubator at 60±1°C for 72 hours, and then dry it in an oven at 80°C for 6 hours to obtain rapeseed cake S2. The measurement results of the protein solubility of the obtained rapeseed cake S2 are listed in Table 1.
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