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Separation and purification method of bacopa monnieri total saponin extract and application of bacopa monnieri total saponin extract in preparation of nervous system drugs

A technology for the separation and purification of Bacopa monniera, applied in the preparation of nervous system drugs, the field of separation and purification of total saponins of Bacopa monniera, can solve the lack of scientific theoretical guidance and Bacopa monnieri foundation for clinical application Insufficient research, unclear material basis of drug efficacy, etc., to achieve the effect suitable for industrial production, large clinical application prospects, and large application value

Inactive Publication Date: 2018-03-13
山东颐正达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Bacopa monnieri has not been developed into any form of medicine so far
The main reason is that the basic research on Bacopa monnieri is insufficient, the material basis of its medicinal effect is not clear, and its pathway of action is not clear, resulting in the lack of scientific theoretical guidance for clinical application and only relying on traditional experience

Method used

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  • Separation and purification method of bacopa monnieri total saponin extract and application of bacopa monnieri total saponin extract in preparation of nervous system drugs
  • Separation and purification method of bacopa monnieri total saponin extract and application of bacopa monnieri total saponin extract in preparation of nervous system drugs
  • Separation and purification method of bacopa monnieri total saponin extract and application of bacopa monnieri total saponin extract in preparation of nervous system drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0043] Example 1-1 Bacopa monniera total saponin content detection method

[0044] (1) Instrument

[0045] Agilent-1200 high performance liquid chromatography (Agilent, USA); KS5200H ultrasonic cleaner (Shanghai Kedao Ultrasonic Instrument Co., Ltd.); BP211D electronic balance (Sartorius, Germany); Milli-Q50SP pure water system (Millipore, USA).

[0046] (2) Reagents

[0047] Acetonitrile (chromatographically pure, Merck Company); phosphoric acid (chromatographically pure, Tedia Company); ultrapure water was made by Milli-Q pure water machine (Merk Millipore); other reagents were of analytical grade.

[0048] (3) Test products and standard products

[0049] Bacopaside I (purity 95%), Chromadex, purchased from Shenzhen Rainbow Xianggen Biotechnology Co., Ltd.), Bacoside A 3 , Bacopaside II, Bacopasaponin C isomer and Bacopasaponin C are made by the laboratory (purity 95%), after MS, 1 H-NMR, 13 C-NMR measurement, to determine its chemical structure, the specific spectral d...

Embodiment 1-2

[0065] Embodiment 1-2 prepares Bacopa monnieri total saponins extract

[0066] Take 5kg of Bacopa monniera medicinal material and place it in a multifunctional extraction tank, add 50kg of 80% ethanol each time, extract 3 times, each time for 1h, combine the extracts, concentrate (pressure -0.6Bar, 60°C) until there is no alcohol smell , add 95% ethanol to adjust the alcohol content to 30%, centrifuge, filter with suction, and the content of the medicinal material is 0.311g·mL -1 of the sample solution. Take the HP-20 macroporous resin that has been pretreated (washed with 95% ethanol 10 times the weight of the resin), and pack it into a column by wet method (diameter-to-height ratio 1:10), with a column volume of 500 mL. Take 800mL of sample solution (0.5kg medicinal material / L resin), adjust the pH value to 2.0 with concentrated hydrochloric acid, and use 0.5BV·h -1 Load the sample at a flow rate until it is complete, then use 2BV of 30% ethanol (pH 2.0) and 5BV of 40% eth...

Embodiment 1-3

[0068] Embodiment 1-3 prepares Bacopa monnieri total saponins extract

[0069] Take by weighing 5 kg of Bacopa monniera medicinal material and place it in a multi-functional extraction tank, add 60 kg of 80% ethanol at a time, extract 2 times, each 2h, combine the extracts, concentrate (decompression, pressure-0.6Bar60°C) to nothing Alcoholic taste, add 95% ethanol to adjust the alcohol content to 30%, centrifuge, filter with suction, and make the medicinal material with a content of 0.311g·mL -1 of the sample solution. Take the HP-20 macroporous resin that has been pretreated (same as above), and pack it into a column by wet method (diameter-to-height ratio 1:10), with a column volume of 500 mL. Take 800mL of the sample solution, adjust the pH value to 2.0 with concentrated hydrochloric acid, and use 0.5BV h -1 Load the sample at a flow rate until it is complete, then use 2BV of 30% ethanol (pH 2.0) and 5BV of 40% ethanol (pH 2.0) to remove impurities at a flow rate of 2.5B...

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Abstract

The invention provides a separation and purification method of a bacopa monnieri total saponin extract. The method comprises the following steps: adding alcohol (80%) into bacopa monnieri, carrying out extraction, merging extracts, concentrating the merged extract; adding alcohol (95%) into the concentrate to prepare a loading solution with a medicinal material content of 0.311 g / mL; using HP-20 macroporous resin to carry out wet process packing and loading until all loading solution is loaded, collecting the eluent, and carrying out reduced pressure distillation to obtain a bacopa monnieri total saponin extract. The purity of total saponins is 51.19 to 55.67%, and the total saponin transfer rate is 76.96 to 78.29%. The invention provides an application of the bacopa monnieri total saponinextract in the preparation of nervous system drugs including drugs for treating depression and dementia. The toxic and side effect of chemical drugs is overcome, and the bacopa monnieri total saponinextract has a wide application prospect in clinic. The separation and purification method adopts macroporous resin to separate and purify the bacopa monnieri total saponin extract; the operation is simple, and the method is suitable for industrial production and has a great application value.

Description

technical field [0001] The invention relates to traditional Chinese medicines, in particular to traditional Chinese medicine extracts, in particular to a separation and purification method of bacopa monnieri total saponins extract and its application in the preparation of nervous system medicines. Background technique [0002] Bacopa monnieri is the whole herb of Bacopamonnieri (L.) Wettst, a plant of the family Scrophulariaceae, also known as bacopa monnieri. It has the effects of clearing away heat and cooling blood, detoxifying and reducing swelling. Clinically, it can also be used to treat respiratory diseases such as bronchitis and asthma. In the Indian Ayurvedic system of medicine, the plant is also believed to have the effects of enhancing intelligence and improving memory, delaying aging, preventing dementia, calming, hypnotizing, and antidepressant. Preparations made from its standard extracts have been marketed in India as an auxiliary drug for the treatment of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/80A61P25/24A61P25/28
CPCA61K36/80A61K2236/333A61K2236/39A61K2236/55
Inventor 张卫东孙青
Owner 山东颐正达医药科技有限公司
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