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Method used for separating and measuring 2-chloromandelic acid enantiomers via HPLC

A kind of technology of o-chloromandelic acid and enantiomer, applied in the field of medicine and chemical industry, and can solve the problems such as the inability to achieve drug effect and the like

Inactive Publication Date: 2018-03-13
WUHAN WUYAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Pharmacological studies have shown that for drugs with chiral enantiomers, only one enantiomer has the desired pharmacological effect, while the other cannot achieve the desired pharmacological effect.

Method used

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  • Method used for separating and measuring 2-chloromandelic acid enantiomers via HPLC
  • Method used for separating and measuring 2-chloromandelic acid enantiomers via HPLC
  • Method used for separating and measuring 2-chloromandelic acid enantiomers via HPLC

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Embodiment 1

[0036] A method for separating and determining o-chloromandelic acid enantiomers by HPLC, comprising the steps of:

[0037] (1) For the preparation of the detection solution:

[0038] Preparation of system suitability solution: Take about 10 mg of o-chloromandelic acid racemate reference substance, accurately weigh it into a 25mL volumetric flask, dissolve it with a diluent and dilute to the mark, shake well, and use it as a system suitability solution.

[0039] Preparation of standard solution: Take about 10 mg of R-o-chloromandelic acid standard, accurately weigh it into a 25mL volumetric flask, dissolve it ultrasonically with a diluent and dilute to the mark, shake well, and use it as a standard solution.

[0040] Preparation of the test solution: Take an appropriate amount of self-made R-o-chloromandelic acid (for the specific production method, refer to the patent "A Method and Application of Enzymatic Preparation of R-o-chloromandelic acid", application number: 201510978...

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Abstract

The invention discloses a method used for separating and measuring 2-chloromandelic acid enantiomers via HPLC. The method comprises following steps: R-2-chloromandelic acid samples are subjected to separating measuring via HPLC, wherein in separating measuring, cellulose-tri-4-methyl benzoate_chiral chromatographic column is taken as a stationary phase chiral chromatographic column, a mixed solution of alkanes and low alcohols is taken as a mobile phase for isocratic elution. The method is capable of realizing separating and measuring 2-chloromandelic acid enantiomers simply, rapidly, and accurately.

Description

technical field [0001] The invention belongs to the field of medicine and chemical industry, and more specifically relates to a method for separating and measuring o-chloromandelic acid enantiomers by HPLC. Background technique [0002] O-chloromandelic acid, the chemical name is 2-(2-chlorophenyl)-2-hydroxyacetic acid, and its R-type and S-type structural formulas are as follows: [0003] [0004] There are chiral properties in life in nature, which leads to the problem of chirality in drugs. Pharmacological studies have shown that for drugs with chiral enantiomers, only one enantiomer has the desired pharmacodynamic effect, while the other cannot achieve the desired pharmacodynamic effect. Consequently, the demand for single enantiomer drugs continues to grow worldwide. In the preparation process of the new anti-platelet aggregation drug clopidogrel (widely used clinically to prevent myocardial infarction, stroke, arteriosclerosis and other cardiovascular and cerebrov...

Claims

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Application Information

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IPC IPC(8): G01N30/74G01N30/06
CPCG01N30/74G01N30/06
Inventor 皮金红赵涛涛夏静平张伟邓军张琦
Owner WUHAN WUYAO PHARMA