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Preparation method of edible-fungus liquid strain culture medium
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A technology of liquid strains and culture medium, applied in botany equipment and methods, liquid fertilizers, applications, etc., can solve the problems of insoluble natural organic components, different sterilization effects, pollution and scrapping, etc.
Inactive Publication Date: 2018-03-16
翔天科技股份有限公司
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[0003] However, the biggest problem facing the liquid cultivation of edible fungi is that the natural organic components of the medium cannot be dissolved in water, thus forming a stratification between the solid phase and the liquid phase of the medium, resulting in the difference in temperature penetration during sterilization and the separation of different phase materials. The sterilization effect is different, and even local sterilization is not complete, which leads to the contamination and scrapping of the whole tank during the cultivation process, which seriously affects the implementation of the liquid strain plan and the subsequent production plan
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[0022] The culture medium of embodiment 1-3 is as shown in table 1
[0023] The medium component of table 1 embodiment 1-3
[0024]
[0025] The preparation method of embodiment 1-3 comprises the following steps,
[0026] (1) Heat the water in the fermenter to 100°C for 1.5 hours;
[0027] (2) Drain the heated water in step (1) into the mixer, and add the culture medium while stirring, the stirring speed is 200rpm, and the duration of the whole process is 15min; the volume ratio of culture medium and water is 1:50.
[0028] (3) The liquid culture medium obtained in step (2) is sterilized at 123°C with a pressure of 0.123MPa;
[0030] (5) Inoculate hair bacteria by conventional methods.
Embodiment 2
[0034] The full bag time of Example 2 was 3 days earlier than that of Comparative Example 2, and the biotransformation rate was 7.6 percentage points higher.
[0036] The culture medium of embodiment 4-6 is as shown in table 2
[0037] The medium component of table 2 embodiment 4-6
[0038]
[0039] The preparation method of embodiment 4-6 comprises the following steps,
[0040] (1) Heat the water in the fermenter to above 90°C, which is 93°C in this example, and keep it for 2 hours;
[0041] (2) Drain the heated water in step (1) into the mixer, add the culture medium while stirring, the stirring speed is 250rpm, and the duration of the whole process is 23min; the volume ratio of culture medium and water is 1:55.
[0042] (3) The liquid culture medium obtained in step (2) is sterilized at 122°C, and the sterilization pressure is 0.12 MPa;
[0044] (5) Inoculate hair bacteria by conventional methods.
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Abstract
The invention relates to a preparation method of an edible-fungus liquid strain culture medium. The preparation method comprises the following steps: (1) heating water in a fermentation tank to 90 DEGC or above, and maintaining the temperature for 1-2 hours; (2) discharging the heated water in the step (1) into a stirrer, and adding a culture medium while stirring, wherein the duration time of the whole process is longer than 10 min and shorter than 30 min; (3) sterilizing the liquid strain culture medium obtained in the step (2) at a temperature of 122-125 DEG C; and (4) carrying out coolingto room temperature. The invention provides the preparation method of the edible-fungus liquid strain culture medium, so that solid-liquid layering of the culture medium is prevented, the sterilization efficiency is improved, thorough sterilization is ensured, and the implementation of a production plan of the edible-fungus liquid strain is ensured.
Description
technical field [0001] The invention belongs to the technical field of liquid strains, in particular to a preparation method of liquid strain culture medium of edible fungi. Background technique [0002] At present, there are two main production methods of edible fungus strains, one is solid culture and the other is liquid culture. Solid culture is the use of traditional solid raw materials such as woody, herbaceous, etc. for solid fermentation of edible fungi, and the obtained solid strains are used for cultivation, and finally fruit bodies are obtained. Liquid culture is the production of strains by using liquid substrate fermentation on the strain culture medium. Edible fungusliquid culture is also called submerged fermentation or submerged culture or liquid fermentation. Its principle is: in a fermenter or a triangular flask, a liquid medium is used, and sterile air is introduced and stirred or oscillated to increase the dissolved oxygen content in the medium. , provi...
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