Preparation method of edible-fungus liquid strain culture medium

Abstract

The invention relates to a preparation method of an edible-fungus liquid strain culture medium. The preparation method comprises the following steps: (1) heating water in a fermentation tank to 90 DEGC or above, and maintaining the temperature for 1-2 hours; (2) discharging the heated water in the step (1) into a stirrer, and adding a culture medium while stirring, wherein the duration time of the whole process is longer than 10 min and shorter than 30 min; (3) sterilizing the liquid strain culture medium obtained in the step (2) at a temperature of 122-125 DEG C; and (4) carrying out coolingto room temperature. The invention provides the preparation method of the edible-fungus liquid strain culture medium, so that solid-liquid layering of the culture medium is prevented, the sterilization efficiency is improved, thorough sterilization is ensured, and the implementation of a production plan of the edible-fungus liquid strain is ensured.

Description

technical field [0001] The invention belongs to the technical field of liquid strains, in particular to a preparation method of liquid strain culture medium of edible fungi. Background technique [0002] At present, there are two main production methods of edible fungus strains, one is solid culture and the other is liquid culture. Solid culture is the use of traditional solid raw materials such as woody, herbaceous, etc. for solid fermentation of edible fungi, and the obtained solid strains are used for cultivation, and finally fruit bodies are obtained. Liquid culture is the production of strains by using liquid substrate fermentation on the strain culture medium. Edible fungus liquid culture is also called submerged fermentation or submerged culture or liquid fermentation. Its principle is: in a fermenter or a triangular flask, a liquid medium is used, and sterile air is introduced and stirred or oscillated to increase the dissolved oxygen content in the medium. , provi...

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Problems solved by technology

[0003] However, the biggest problem facing the liquid cultivation of edible fungi is that the natural organic components of the medium cannot be dissolved in water, thus forming a stratification between the solid phase and the liquid phase of the medium, resulting in the difference in temperature penetration during sterilization and the separation of different phase materials. The sterilization effect is different, and even local sterilization is not complete, which leads to the contamination and scrapping of the whole tank during the cultivation process, which seriously affects the implementation of the liquid strain plan and the subsequent production plan

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