Ectropis obliqua nuclear polyhedrosis virus and Beauveria bassiana powder compounded insecticidal suspension, and preparation method and application thereof
A technology of nuclear polyhedron and Beauveria bassiana, applied in the direction of pesticides, botanical equipment and methods, applications, etc., can solve problems such as rampant pests, increased pesticide residues in tea, and destruction of ecological balance
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[0034] In the present invention, the preparation method of the tea geometrid nuclear polyhedrosis virus suspension comprises the following steps:
[0035] 1) inoculate the tea geometrid nuclear polyhedrosis virus into the tea geometrid, and obtain the carcass after infection;
[0036] 2) mixing the insect corpses obtained in the step 1) with water, mashing and filtering to obtain the filtrate;
[0037] 3) centrifuging the filtrate obtained in step 2) at 400-600 rpm for 3-8 minutes to obtain a supernatant;
[0038] 4) centrifuging the supernatant obtained in step 3) at 3000-4000 rpm for 25-35 minutes to obtain a precipitate;
[0039] 5) Mix the precipitate obtained in the step 5) with water to obtain a tea geometrid nucleopolyhedrosis virus suspension.
[0040] In the present invention, the tea geometrid in the step 1) is preferably tea geometrid larvae, and the age of the tea geometrid larvae is preferably 3-5 instars, more preferably early 4th instars. In the present inven...
Embodiment 1
[0083] Preparation of Tea Geometrid Nuclear Polyhedrosis Virus Suspension:
[0084] The tea geometrid nuclear polyhedrosis virus was inoculated into the tea geometrid larvae, and the carcass were obtained after infection. After the carcass was added with deionized water, it was mashed by a high-speed tissue grinder and filtered with two layers of gauze. The filtrate was centrifuged at 500rpm for 5 minutes. The supernatant was obtained, and the supernatant was centrifuged at 3000rpm for 30min to obtain a precipitate, which was diluted with deionized water to obtain a tea geometrid nucleopolyhedrosis virus suspension, and the content of the tea geometrid nucleopolyhedrosis virus was 2.0× 10 10 PIB / mL.
[0085] Preparation of Beauveria bassiana powder:
[0086]Inoculate the preserved Beauveria bassiana species into an eggplant-type bottle equipped with nutrient agar medium, and cultivate it at 26°C for 9 hours for activation culture; the activated Beauveria bassiana strain was ...
Embodiment 2
[0088] In terms of mass percent of the total amount of insecticidal suspension concentrate: 10% of the tea geometrid nucleopolyhedron virus suspension prepared in Example 1; 20% of Beauveria bassiana powder prepared in Example 1; 0.2% of sodium benzoate; Silicone defoamer 0.15%; sodium lignosulfonate 2%; magnesium aluminum silicate 1.2%; xanthan gum 0.2%; glycerin 2%; deionized water to make up 100%.
[0089] Preparation method: first add Beauveria bassiana powder, sodium lignosulfonate, magnesium aluminum silicate, xanthan gum, glycerin, sodium benzoate, silicone defoamer and deionized water in sequence, and then add the colloidal virus suspension; use a colloid mill to uniformly grind the above mixture so that 98% of the particle size is below 75 μm to obtain a colloidal grinding solution; the colloidal grinding solution is ground by a sand mill so that 98% of the particle size is below 5 μm to obtain sand Grinding liquid; adjust the pH value of the sanding liquid to 6.0 to ...
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