Combined preparation for nanoparticle tumor diagnoses and treatment and preparation method and application thereof

A nanoparticle and tumor diagnosis technology, which is applied in the field of biomedical materials and tumor diagnosis and treatment preparations, can solve the problems of poor targeting function, low drug loading, high immunogenicity, etc., and achieve extended half-life, good biological safety, and reduced Effects of Laser Energy

Active Publication Date: 2018-03-27
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nanocarrier systems such as microcapsules, liposomes, and polymer micelles that have been studied more now generally have problems such as high immunogenicity, low drug loading, easy drug leakage, poor targeting function, and easy aggregation.

Method used

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  • Combined preparation for nanoparticle tumor diagnoses and treatment and preparation method and application thereof
  • Combined preparation for nanoparticle tumor diagnoses and treatment and preparation method and application thereof
  • Combined preparation for nanoparticle tumor diagnoses and treatment and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The preparation process of the nano-particle tumor diagnostic and therapeutic combination preparation of the present invention (the diagnostic and therapeutic preparation of Prussian blue nanoparticles that efficiently adsorb the radioactive rare earth element lutetium) is as attached figure 1 As shown, taking Prussian blue nanoparticles, radioactive rare earth element lutetium and platelet membrane as an example, the description includes the following steps:

[0040] (1) Preparation of citric acid-modified Prussian blue nanoparticles by hydrothermal method: heating and stirring the citric acid solution of ferric salt and ferric salt in a water bath and then cooling to room temperature to obtain a dark blue liquid. After centrifugal cleaning, put it into freezing Freeze-dried in the dryer into freeze-dried powder. Adjust the preparation process to make the prepared nanoparticles have a particle size of about 100 nm, uniform size, and good dispersion.

[0041] (2) Adsorption ...

Embodiment 2

[0044] The characterization data of the nanoparticle tumor diagnostic and therapeutic combination preparation of the present invention (the diagnostic and therapeutic preparation of Prussian blue nanoparticles that efficiently adsorb the radioactive rare earth element lutetium) is as follows:

[0045] The peak of the absorption spectrum of the Prussian blue nanoparticle solution modified with citric acid prepared by the hydrothermal method is about 700 nm, which is the characteristic absorption peak of the Prussian blue nanoparticle. The hydration radius is 95.72±3.44 nm, and the surface potential is -27.86±0.90 mV. The aqueous solution of Prussian blue nanoparticles is uniform and stable, and there is no aggregation phenomenon. Observation of the shape and size of the Prussian blue nanoparticles by transmission electron microscope found that the shape is a regular hexahedron with a particle size of about 100 nm. figure 2 Shown.

[0046] The ultraviolet-visible-near-infrared absor...

Embodiment 3

[0048] In vivo SPECT / CT imaging includes the following steps:

[0049] (1) Select the tumor size to be approximately 150 mm 3 Of tumor-bearing nude mice (the tumor model is a human colon cancer cell HT-29 model).

[0050] (2) The concentration of tail vein injection is 10 mg mL -1 100μL of Prussian blue nanoparticles that efficiently adsorb the radioactive rare earth element lutetium.

[0051] (3) Monitoring the SPECT / CT imaging of nanoparticles in 24 hours by SPECT / CT.

[0052] The experimental results are attached Figure 5 As shown, 24 hours after drug injection, SPECT / CT imaging light signals can be detected in the tumor site of nude mice. This indicates that nanoparticles can accumulate in a large number of tumor sites in nude mice, and the tumor sites can be imaged by SPECT.

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Abstract

The invention discloses a combined preparation for nanoparticle tumor diagnoses and treatment and a preparation method and an application thereof. The preparation adsorbs radioactive rare earth element lutetium ions with prussian blue nanoparticles which are good in biocompatibility and platelet membranes coat the surfaces of the nanoparticles. The prussian blue nanoparticles used in the preparation can be used for photo-acoustic imaging and generates energy through near infrared laser irradiation while monitoring a diagnoses and treatment agent in real time so as to improve the temperature tokill tumor cells for photothermal therapy. Meanwhile, the radioactive rare earth element lutetium ions also can realize SEPCT imaging and radiotherapy functions. The platelet membranes coating the surfaces of the nanoparticles can improve the recognition of tumor tissues so as to realize a function of targeting tumors. In addition, the nano diagnoses and treatment preparation is high in biosafety, good in tumor targeting property, simple in preparation method, low in cost and good in repeatability, and has wide application prospect in the field of tumor diagnoses and treatment.

Description

Technical field [0001] The invention relates to the technical field of biomedical materials, in particular to the technical field of tumor diagnosis and treatment preparations, and in particular to a nanoparticle tumor diagnosis and treatment combination preparation and a preparation method thereof. Background technique [0002] In recent years, the research on tumor treatment has become a hot research topic in materials chemistry, biophysics, pharmacy, radiology, medical imaging and other disciplines, but its clinical treatment effect is still not ideal. In order to better treat tumors, it is necessary to apply the latest technical methods and design concepts of materials science, physics and biomedicine, design intelligent targeted drug carriers, and conduct real-time detection through technical imaging methods of medical imaging, and Various treatment methods are used in combination to better treat tumors. [0003] Radiotherapy is a local treatment method that uses radiation t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K51/12A61K51/04A61K51/02A61K41/00A61P35/00A61K103/30
CPCA61K41/0052A61K49/221A61K49/225A61K51/02A61K51/04A61K51/1251
Inventor 王凡李小达梁晓龙史继云贾兵马晓途高瀚男张欣姚美男邵楠张旭侯睿
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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