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Magnesium hyaluronate for treating osteoarthritis and preparation method and application thereof

A technology for magnesium hyaluronate and osteoarthritis, which is applied to medical preparations containing active ingredients, anti-inflammatory agents, and pharmaceutical formulas, and can solve problems such as inaccurate efficacy of intra-articular injection of sodium hyaluronate

Inactive Publication Date: 2018-03-27
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the latest evidence-based medical evidence shows that the curative effect of intra-articular injection of sodium hyaluronate is inaccurate, and the three major international authoritative OA guidelines do not recommend intra-articular injection of sodium hyaluronate (Hochberg et al., Arthritis Care Res( Hoboken, 2012, 64:465‐474; McAlindon et al., Osteoarthritis Cartilage, 2014, 22:363‐388; Brown et al., J AmAcad Orthop Surg, 2013, 21:577‐579)

Method used

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  • Magnesium hyaluronate for treating osteoarthritis and preparation method and application thereof
  • Magnesium hyaluronate for treating osteoarthritis and preparation method and application thereof
  • Magnesium hyaluronate for treating osteoarthritis and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of Magnesium Hyaluronate

[0026] 1. Take 300mg of magnesium acetate and dissolve it completely in 5ml of distilled water.

[0027] 2. Put 100mL of ethanol into the magnesium acetate solution, stir evenly, and heat to reach 40°C.

[0028] 3. While stirring, add 500 mg of sodium hyaluronate and react at a temperature of 40°C for 60 minutes.

[0029] 4. After the reaction is over, let it stand for 15 minutes, pour off the supernatant, and centrifuge to obtain a precipitate.

[0030]5. Take the precipitate, add 90% (v / v) ethanol with 5 times the mass of the precipitate, stir for 10 minutes, let stand for 15 minutes, and centrifuge to obtain the precipitate.

[0031] 6. Step 5 was repeated 5 times, and the obtained precipitate was vacuum-dried at 60° C. to obtain 450 mg of magnesium hyaluronate, and the yield (%) of magnesium hyaluronate = 450 / 500 = 90%. The structure is as formula (Ⅰ):

[0032]

Embodiment 2

[0033] Example 2 In vitro cytotoxicity evaluation of magnesium hyaluronate for chondrocytes

[0034] 1 Experimental materials and methods

[0035] 1.1 Drugs and reagents

[0036] Magnesium Hyaluronate, Sodium Hyaluronate, Normal Saline

[0037] Cell Counting Kit (CCK-8 Kit)

[0038] 1.2 cells

[0039] mouse chondrocytes

[0040] 1.3 Experimental method

[0041] 1.3.1 Take mouse chondrocytes growing in the logarithmic phase, inoculate 5000 cells / well in 96-well culture plate, each well volume is about 100 μl, in 5% CO 2 , 37°C incubator for about 24h until the cells grow to a monolayer. Then, discard the supernatant in the well, wash with phosphate buffered solution (PBS) for 1-2 times, add complete medium (DMEM / F12+10% fetal bovine serum) and a series of drug solutions (100 μl ), were divided into 5 groups, and each group had 3 replicate wells. The cell plate was further incubated in the incubator for 48 h. Grouped as follows:

[0042] 1) 5ng / ml magnesium hyaluronate...

Embodiment 3

[0052] Example 3 Preparation of magnesium hyaluronate intra-articular injection

[0053] At room temperature, dissolve the magnesium hyaluronate synthesized according to Example 1 with physiological saline, and dilute to a corresponding concentration of 1%, filter through a 0.22 μm filter membrane, potting, sterilizing, inspecting and packaging.

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Abstract

The invention discloses magnesium hyaluronate for treating osteoarthritis and a preparation method and application thereof. The preparation method of the magnesium hyaluronate comprises adding a magnesium salt aqueous solution into an appropriate amount of a sodium hyaluronate aqueous solution for precipitation so that an associated complex of hyaluronic acid and magnesium ions is obtained. A testresult shows that compared with the sodium hyaluronate, the drug magnesium hyaluronate can well delay cartilage degeneration, so as to achieve better treatment on osteoarthritis.

Description

technical field [0001] The invention relates to a medicine for treating osteoarthritis and a preparation method thereof, in particular to magnesium hyaluronic acid salt for treating osteoarthritis, a preparation method and application thereof. technical background [0002] Osteoarthritis (OA) is a joint degenerative disease characterized by degeneration of articular cartilage, subchondral bone sclerosis and osteophyte formation, and is mainly characterized by joint pain, limited mobility and joint deformation after activities. Clinical manifestations, often involving weight-bearing joints. Epidemiological surveys in my country show that the prevalence of OA in people over 65 years old exceeds 50%, about 80% of OA patients have a certain degree of movement limitation, and 25% of OA patients are significantly affected in daily life. The cost of treating OA may be as high as 150 billion yuan. Among people over 50 years old in the United States, the disability rate of OA ranks ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K31/728A61K33/06A61K45/06A61P19/02A61P29/00
CPCA61K31/726A61K31/728A61K33/06A61K38/39A61K45/06C08B37/0072A61K2300/00
Inventor 雷光华曾超刘韶李辉杨拓崔洋
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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