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Method for rapidly extracting benzo[a]pyrene in food

An extraction method, food technology, applied in separation methods, chemical instruments and methods, preparation of test samples, etc., can solve the problems of unsatisfactory detection, large amount of organic solvent, cumbersome operation, etc., and achieve accurate and rapid qualitative and quantitative detection, The effect of less solvent consumption, simple and fast operation

Inactive Publication Date: 2018-03-27
DALIAN ELITE ANALYTICAL INSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing extraction method of benzo[a]pyrene in food is solid-phase extraction, that is, the sample is purified by using a solid-phase extraction column to extract benzo[a]pyrene in food. Large size, long time-consuming and other disadvantages, when there are many samples, it cannot meet the detection requirements

Method used

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  • Method for rapidly extracting benzo[a]pyrene in food
  • Method for rapidly extracting benzo[a]pyrene in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] a. After drying the instant noodles, pulverize and homogenize them with a homogeneous disperser to obtain samples for later use;

[0012] b. Add 300 mg tetrabutylammonium bromide, 100 mg octanol and 8 mL distilled water to a 10ml centrifuge tube in sequence, mix well and centrifuge at 8000r / min for 3min to make supramolecular solvent phase and water phase layer up and down, remove with a syringe The lower aqueous phase, the upper supramolecular solvent is preserved in a sealed bottle;

[0013] c. Mix 200 mg of the sample obtained in step a with 800 µL of the supramolecular solvent obtained in step b, and ultrasonically treat at room temperature at 20KHz for 10 minutes, then pass the mixture through a 0.45 μm organic filter membrane, and take the filter membrane liquid.

Embodiment 2

[0015] a. After drying the fried dough sticks, pulverize and homogenize them with a homogeneous disperser to obtain samples for later use;

[0016] b. Add 300 mg tetrabutylammonium hydroxide, 100 mg heptanol and 8 mL distilled water to a 10 ml centrifuge tube in sequence, mix well and centrifuge at 5000 r / min for 8 min to separate the supramolecular solvent phase and the water phase, and remove the lower layer of water with a syringe Phase, the upper supramolecular solvent is stored in a sealed bottle;

[0017] c. Mix 400 mg of the sample obtained in step a with 1000 µL of the supramolecular solvent obtained in step b and ultrasonically treat at room temperature at 40KHz for 5 minutes, then pass the mixture through a 0.45 μm organic filter membrane, and take the filter membrane liquid.

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Abstract

The invention discloses a method for rapidly extracting benzo[a]pyrene in food. The method comprises the following steps: drying food, and crushing the food by a homogeneous disperser and performing homogenization to obtain a sample for standby; adding a component A, a component B and distilled water in a centrifuge tube in order, uniformly mixing the materials and performing centrifugation for 3-10 min at 3000-8000 r / min so that a super molecular solvent phase and a water phase are in an up-down structure, removing the water phase at a lower layer, and keeping an upper layer super molecular solvent; wherein the mass ratio of the component A to the component B to distilled water is 1:0.2-0.4:5-10, the component A is at least one of tetrabutyl ammonium hydroxide, tetrabutylammonium bromideand tetrabutylammonium hydrogen sulfate, the component B is at least one of heptanol, octanol and nonanol, mixing the sample in the step a) and the super molecular solvent in the step b) and performing ultrasonic processing for 5-20 min under 20-40 KHz, then passing the mixed liquor through an organic filter membrane with 0.45 [mu]m, and taking the filter membrane liquid.

Description

technical field [0001] The invention relates to a food detection technology, in particular to a quick extraction method of benzo[a]pyrene in food which is time-saving, labor-saving, easy to operate and environment-friendly. Background technique [0002] Benzo[a]pyrene is an organic compound with obvious carcinogenic effect among polycyclic aromatic hydrocarbons. Cancer, etc., is a first-class carcinogen. In recent years, the food safety problem caused by benzo[a]pyrene has also been paid more and more attention by people. my country's national standard limits the residual amount of benzo[a]pyrene in grain and meat products to be below 5 μg / kg, and 10 μg in vegetable oil. / kg or less. At present, the determination methods of benzo[a]pyrene content in food mainly include fluorescence spectrometry, enzyme-linked immunosorbent assay, gas chromatography-mass spectrometry and high-performance liquid chromatography, and most of the measurement methods require the detection of Foo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N1/40G01N30/06B01D11/02
CPCB01D11/0238G01N1/34G01N1/4055G01N30/06G01N2001/4061G01N2030/062
Inventor 谈义萌唐涛杨三东夏明珠王风云李彤
Owner DALIAN ELITE ANALYTICAL INSTR
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