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Antioxidant application of apios Americana medikus leaf polysaccharides

An anti-oxidation and bean taro technology, applied in the field of medicine, can solve the problems of rare reports on the antioxidant activity of bean taro leaf extracts, achieve good development prospects, expand application fields, and reduce the production of active oxygen

Active Publication Date: 2018-04-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, many nutrition and health food researchers are currently paying great attention to research and topics related to anti-oxidation and the occurrence and prevention of chronic diseases, but there are few reports on the antioxidant activity of bean taro leaf extract

Method used

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  • Antioxidant application of apios Americana medikus leaf polysaccharides
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  • Antioxidant application of apios Americana medikus leaf polysaccharides

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Embodiment 1, a kind of preparation method of bean taro leaf polysaccharide, carries out following steps successively:

[0060] 1), the preparation of the crude product of bean taro leaf polysaccharide:

[0061] According to the solid-liquid ratio of 1g:10ml, weigh 100g of taro leaf and add 1000mL distilled water, extract in 80°C water bath for 4h (enzyme inactivation is also achieved during extraction), then beat, and then centrifuge (4000r / min for 30min), and Suction filter the supernatant obtained by centrifugation and collect the filtrate;

[0062] Replace the taro leaf with the filter residue obtained by centrifugation, repeat the above process of adding distilled water, then extracting (4h in 80°C water bath), beating, and centrifuging twice, and combine the filtrate obtained by centrifugation and suction for three times and concentrate to the original volume by rotary evaporation 1 / 5 of the concentrated solution.

[0063] Under stirring conditions, add ethanol ...

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Abstract

The invention discloses antioxidant application of apios Americana medikus leaf polysaccharides. The antioxidant application of the apios Americana medikus leaf polysaccharides is applied to preparinghypatocyte oxidative damage inhibitor. The preparation method of the apios Americana medikus leaf polysaccharides comprises 1) preparing coarse apios Americana medikus leaf polysaccharides by mixingapios Americana medikus leaves with distilled water for extraction, and performing pulping, centrifugation and concentration and adding ethyl alcohol into obtained concentrated solution until reachingan ethyl alcohol concentration of 80%-90%, standing the mixed solution at 3-5 DEG C for 22-26 hours and collecting precipitates; 2) purifying the apios Americana medikus leaf polysaccharides by performing activated carbon decoloring and deproteinization on the coarse apios Americana medikus leaf polysaccharides.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of bean taro leaf polysaccharide and its anti-oxidation application. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitamin E and minerals. Among them, taro le...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003
Inventor 郑晓冬楚强李永璐余露霜刘阳阳
Owner ZHEJIANG UNIV
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