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Special fermentation microbial inoculum for sugarcane by-product feed and preparation method thereof

A technology for fermentation inoculum and by-products is applied in the field of special fermentation inoculum for sugarcane by-product feed and its preparation, which can solve the problems of the threat to the healthy development of herbivorous animal husbandry, the waste of sugarcane by-products, and the single utilization mode, so as to improve the nutritional value. , good development prospects and social significance, good stability

Pending Publication Date: 2019-05-14
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to overgrazing, deforestation and deforestation, grassland degradation is serious, and the healthy development of herbivorous animal husbandry is seriously threatened
Although China is a big sugarcane planting country, the utilization mode of sugarcane by-products is still relatively simple, which contains a large amount of crude fiber that is difficult for animals to digest and absorb. It is still difficult to develop sugarcane by-product feed products, and it is difficult to carry out comprehensive promotion. It caused a lot of waste of sugarcane by-products, especially bagasse. Many sugar companies can only use the bagasse after squeezing sugarcane juice for papermaking or burning, which cannot be fully utilized, resulting in great waste.

Method used

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  • Special fermentation microbial inoculum for sugarcane by-product feed and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The domestication method of domestication bacterium comprises the following steps:

[0046] (1) Selection of bacteria slime: Select sugarcane leaf retting sludge for more than half a year, and sludge formed by stacking bagasse stacking area of ​​sugar factory for more than half a year, and mix according to the weight ratio of 1:1 to get the original bacteria sludge, and put the original sludge on site Seal it in a sterile glass bottle and bring it back to the laboratory for cultivation as the original flora;

[0047] (2) Primary domestication: take fresh sugarcane tails and crush them to 200 mesh, autoclave at 121°C for 15 minutes, add them to the prepared basic medium after cooling, and mix them evenly to obtain a primary domestication medium, sugarcane tails and basic culture The weight ratio of base is 1.0~1.5:1;

[0048] According to the difference in the amount of sugarcane tails added, 6 gradients were set: 1.0:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1, 1.5:1, and 6 kinds of...

Embodiment 2

[0067] The special fermented bacterial agent for sugarcane by-product feed includes the following raw materials in parts by weight: 30 parts of freeze-dried powder of domesticated bacteria, 8 parts of freeze-dried powder of Candida utilis, 6 parts of freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Bacillus subtilis 8 parts of powder, 4 parts of Aspergillus niger freeze-dried powder, 4 parts of Aspergillus oryzae freeze-dried powder, 7 parts of Trichoderma viride freeze-dried powder, 18 parts of cellulase freeze-dried powder;

[0068] The preparation method of the freeze-dried powder of domesticated bacteria is as follows: take the bacterium liquid that reaches the stable phase during the secondary domesticated bacterium expansion culture, centrifuge, take the bacterium sludge and wash it with sterile phosphate buffer saline, centrifuge and resuspend in sterile phosphoric acid Add embedding agent to salt buffer solution, and carry out vacuum freeze-drying ...

Embodiment 3

[0076] The special fermented bacterial agent for sugarcane by-product feed includes the following raw materials in parts by weight: 22 parts of freeze-dried powder of domesticated bacteria, 9 parts of freeze-dried powder of Candida utilis, 7 parts of freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Bacillus subtilis 9 parts of powder, 5 parts of Aspergillus niger freeze-dried powder, 5 parts of Aspergillus oryzae freeze-dried powder, 8 parts of Trichoderma viride freeze-dried powder, 19 parts of cellulase freeze-dried powder;

[0077] The preparation method of the freeze-dried powder of domesticated bacteria is as follows: take the bacterium liquid that reaches the stable phase during the secondary domesticated bacterium expansion culture, centrifuge, take the bacterium sludge and wash it with sterile phosphate buffer saline, centrifuge and resuspend in sterile phosphoric acid Add embedding agent to salt buffer solution, and carry out vacuum freeze-drying ...

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Abstract

The invention discloses a special fermentation microbial inoculum for sugarcane by-product feed and a preparation method thereof. The fermentation microbial inoculum comprises special domesticated bacteria freeze-dried powder and freeze-dried powder of various active ingredients such as candida utilis, lactobacillus plantarum, bacillus subtilis, aspergillus niger, aspergillus oryzae, trichoderma viride and the like, which can effectively degrade sugarcane by-products such as sugarcane tail tips, bagasse, molasses and the like, and each strain is coordinated and matched with each other, so thatthe method has the advantages of high fermentation efficiency, high speed, low energy consumption, high conversion rate and good stability. Domestication bacteria used by the fermentation microbial inoculum are from nature, and are obtained through enrichment, screening and domestication, which have super strong propagation and adaptability, can decompose cellulose in sugarcane by-products in a targeted manner, high molecular carbohydrates which are difficult to be fully digested and absorbed by animals such as crude fiber, lignin and the like can be converted into low molecular substances which can be absorbed and utilized, meanwhile, the content of crude protein in fermentation feed is improved, and nutrition and palatability of feed are improved.

Description

technical field [0001] The invention relates to the technical field of feed starter, in particular to a special fermentation starter for sugarcane by-product feed and a preparation method thereof. Background technique [0002] sugar cane( Saccharum of ficinarum L. ) is a perennial grass family ( Graminae ) Tall stalk monocotyledonous plants of the Milo family, widely planted in tropical and subtropical regions. Sugarcane is a C4 plant with a high conversion rate of light energy and a high yield per unit area. It is an economic crop that utilizes solar energy efficiently. Sugarcane contains sufficient water, sugar, protein, fat, organic acids and various vitamins. As an important non-grain feed crop, sugarcane can effectively alleviate the problem of feed shortage in winter and spring. Guangxi is a major sugarcane producing area, and is rich in by-products of the sugar industry such as bagasse, sugarcane tip leaves, molasses, etc. These by-products are large in quantity, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20C12N1/16C12N1/14C12N9/42A23K10/12A23K10/14A23K10/37A23K50/10C12R1/72C12R1/25C12R1/125C12R1/685C12R1/69C12R1/885
CPCY02P60/87
Inventor 淡明张娥珍蓝桃菊梁晓君王冬梅黄振勇黄梅华何全光韦馨平
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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