A heart chip based on structural color hydrogel and its application

Abstract

A heart chip based on structural color hydrogel comprises a chip top plate (1), a chip bottom plate (2), and a structural color hydrogel membrane (8) on which myocardial cells grow. The chip bottom plate (2) is provided with a liquid storage micro-tank (7). One end of the liquid storage micro-tank (7) is communication with a liquid inlet (3) in the chip bottom plate (2) through flow distribution micro-channels (6), and the other end of the liquid storage micro-tank (7) is communication with a liquid outlet (4) in the chip bottom plate (2), and hydrogel membrane mounting micro-columns (5) are disposed in the liquid storage micro-tank (7) and are used for placing the structural color hydrogel membrane (8). A liquid inlet hole (9) and a liquid outlet hole (10) are formed in the chip top plate (1) and positionally correspond to the liquid inlet (3) and the liquid outlet (4) in the chip bottom plate (2). The heart chip constructed based on a structural color hydrogel material in the present invention does not need a complicated detection system, is intuitive and is not affected by impacts on the detected system in physiological and pharmaceutical aspects and the like; more importantly, the detection method is not affected by impacts of external environments, chemical reagents and other factors, and the heart chip can show the unique intrinsic advantages that the heart chip is sensitive and efficient during screening and evaluation of heart drugs and is not affected by external conditions.

Description

technical field [0001] The invention relates to a heart chip based on structural color hydrogel and its application, and belongs to the field of organ chips. Background technique [0002] The human body is an organism composed of a variety of complex organs, and the interaction between the organs enables humans to achieve a variety of metabolic behaviors in the body. At present, for human drug development, in vitro cell culture and animal in vivo experiments are mainly used to detect the effect of drugs on target objects, so as to determine drug properties; however, the two-dimensional cell structure produced by in vitro cell culture cannot truly simulate the human microenvironment. and complex physiological processes between tissues and organs, while animal in vivo experiments are also constrained by the uncertainty of animal models, the complexity of different animals, and some humanitarian factors; Organ-on-a-chip to simulate real physiological processes has attracted wi...

AI Technical Summary

Problems solved by technology

At present, for the development of drugs for humans, it is mainly through in vitro cell culture and animal in vivo experiments to detect the effect of drugs on target objects, so as to judge the characteristics of drugs; however, the two-dimensional cell structure produced by in vitro cell culture cannot truly simulate the human microenvironment As well as the complex physiological processes between various tissues and organs, while animal experiments are also restricted by the uncertainty of animal models, the complexity of different animals and some humanitarian factors; for this reason, constructing cells or organs at the level of cells or organs Organ-on-a-chip to mimic real physiological processes has attracted widespread interest

[0003] The heart is one of the most important organs of the human body. The heart chip is an important way to realize the simulation of human heart function and detection behavior. However, the heart chip needs complex detection systems after construction, and these detection systems often consume a lot of time and energy. And Lack of intuition; in the research of heart and cardiomyocytes, the contractility and beating frequency of cardiomyocytes are the most important evaluation parameters, which are usually important indicators for judging cardiac function and cardiomyocyte activity; therefore, the research and development of Sensing materials for detecting the contractility and beating frequency of cardiomyocytes with high sensitivity, and the construction of a stable contraction force and beating frequency sensing heart chip and drug research platform are still facing severe challenges

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