Application of water-soluble fullerene structure in preparation of medicine for treating tumors

A fullerene structure, water-soluble technology, applied in the field of biomedicine, can solve problems such as side effects, lack of targeting of chemotherapy drugs, and normal cell death

Inactive Publication Date: 2018-04-17
BEIJING FUNAKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current process of tumor treatment, chemotherapy is one of the most commonly used methods, but chemotherapy drugs lack targeting, and can cause the death of normal cells while killing tumor cells, so they often cause serious side effects

Method used

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  • Application of water-soluble fullerene structure in preparation of medicine for treating tumors
  • Application of water-soluble fullerene structure in preparation of medicine for treating tumors
  • Application of water-soluble fullerene structure in preparation of medicine for treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Embodiment 1, the synthesis of water-soluble fullerene structure

[0089] (1)C 70 (OH) 29 10H 2 Synthesis of O

[0090] C in toluene solution 70 Mix with NaOH solution with a concentration of 50%, and add a small amount of phase transfer catalyst tetramethylammonium hydroxide (TBAH), stir for 24h, add ethanol for the precipitation product, centrifuge to remove the supernatant liquid, and then dissolve the precipitated product collected in a supernatant In pure water, dialyze to remove excess NaOH to obtain product C 70 (OH) 29 10H 2 O, freeze-dried and stored.

[0091] (2)C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 15H 2 Synthesis of O

[0092] A NaOH solution containing serine (the mass fraction of NaOH in the NaOH solution is 33%, and the molar ratio of serine to NaOH is 1:4) is mixed with ethanol, and added dropwise to C 70 (make serine and C 70 In a toluene solution with a molar ratio of 100:1), stir overnight (12h), remove the upper toluene layer, and dialyze t...

Embodiment 2

[0098] Embodiment 2, modifying the number of hydroxyl groups and the number of amino acids in the water-soluble fullerene structure

[0099] (1) Measure the number of hydroxyl groups modified by the water-soluble fullerene structure obtained in part (1) of Example 1

[0100] According to elemental analysis (Flash EA 1112) result: C 53.55%, H 3.19%, Nfigure 1 shown) to calculate the number of hydroxyl groups modified by the water-soluble fullerene structure obtained in part (1) in Example 1. From thermogravimetric analysis, it can be known that C 70 (OH) x The solid powder contains 11.8% water, which is about 10 water molecules. Combined with the H content in the elemental analysis, it is estimated that the H content in the hydroxyl group is 1.87%. From this, the C 70 (OH) x The number of hydroxyl groups is 29, so the average molecular formula of the product is C 70 (OH) 29 10H 2 O, hereafter abbreviated as C 70 (OH) 29 .

[0101] (2) Determination of the number of hyd...

Embodiment 3

[0105] Embodiment 3, the mensuration of the hydrated particle size of water-soluble fullerene nanomaterial

[0106] Get a small amount of C prepared by Example 1 of the present invention 70 (OH) 29 、C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 and C 70 (OH) 24 The powder is dissolved in water to form a dilute solution. Due to the intermolecular interaction, C 70 (OH) 29 , C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 and C 70 (OH) 24 Agglomerates into nanoparticles in water to form particles with a hydrated particle size of 1-200nm.

[0107] Adopt dynamic light scattering (DLS, Zetasizer Nano ZSP) to measure the hydrated particle diameter of nanoparticle in the pure water of pH=7.0, C 70 (OH) 29 、C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 and C 70 (OH) 24 The average particle diameters are 136.4±0.5nm, 134.7±0.4nm and 135.9nm, respectively, see Figure 4 and Figure 5 . C 70 (OH) 29 Zeta potential is -55.6mV, see Figure 6 .

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Abstract

The invention discloses application of a water-soluble fullerene structure in preparation of a medicine for treating tumors. The water-soluble fullerene structure comprises water-soluble hollow fullerene and at least one of pharmaceutical salts and pharmaceutical esters thereof. The medicine has the beneficial effects that the affinity to organisms is good, the toxicity is low, the tumor inhibiting efficiency is high and the tumor treating effect is good.

Description

[0001] The present invention requires Beijing Funakang Biotechnology Co., Ltd. and the Institute of Chemistry, Chinese Academy of Sciences to submit to the Chinese Patent Office on May 9, 2017, the application number is CN201710322042.0, and the invention name is "Water-soluble fullerene nanoparticles in the preparation of The priority of the Chinese patent application "Applications in Inhibiting Tumor Growth Drugs", the entire content of which is incorporated in the present invention by reference. [0002] The present invention requires Beijing Funakang Biotechnology Co., Ltd. to submit to the Chinese Patent Office in August 2016, the application number is CN201610878746.1, and the title of the invention is "a water-soluble fullerene nanomaterial and its preparation method and application", the entire content of which is incorporated in the present invention by reference. technical field [0003] The invention relates to the field of biomedicine, in particular to the applicat...

Claims

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Application Information

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IPC IPC(8): A61K33/44A61K47/54A61P35/00
CPCA61K33/44
Inventor 王春儒甄明明周悦白春礼
Owner BEIJING FUNAKANG BIOTECH CO LTD
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