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Fish ovarian tissue paraffin section making method

A technology for ovarian tissue and paraffin sectioning, which is applied in the preparation of test samples, etc., which can solve the problems that immune and molecular biology analysis cannot be performed, it is not easy to make slices, and the slices are easy to form cavities, etc., so as to increase penetration and fixation effect, improve structure clarity, improve the effect of dehydration

Inactive Publication Date: 2018-04-17
SHANXI AGRI UNIV
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Problems solved by technology

However, fish ovaries are rich in yolk, which makes it difficult to make slices. According to the general method in the past, the slices are fragile, and cavities are easily formed in the middle of the slices, which makes the structure unclear in microscopic observation. Other immunological and molecular biological analyzes cannot conduct

Method used

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Embodiment 1

[0026] The invention is used to make paraffin sections of fish ovary tissue, and to observe the pathological changes and immunohistochemical localization of carp ovary tissue after exposure to different concentrations of copper for 30 days. Specific steps are as follows:

[0027] 1. Preparation of related reagents

[0028] (1) Preparation of Zamboni’s fixative solution Zamboni’s fixative solution is made up of the following reagents, paraformaldehyde 25g, saturated picric acid 200ml, Karasson-Schwlt’s phosphate buffer to 1000ml.

[0029] (2) Prepare paraffin. Purchase paraffin wax with a melting point of 52-56°C in advance.

[0030] (3) Preparation of Harris' hematoxylin solution Harris' hematoxylin solution is prepared from the following reagents: 2g of hematoxylin, 30ml of absolute ethanol, 40g of potassium aluminum sulfate, 500ml of distilled water, 1.5g of red mercuric oxide, and 15ml of glacial acetic acid.

[0031] 2. Paraffin section making

[0032] (1) Material samp...

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PUM

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Abstract

The invention discloses a fish ovarian tissue paraffin section making method which includes the steps of material sampling, tissue fixation, dehydration and transparency, waxing, embedding, slicing, pasting and baking, dewaxing, rehydration, cell nucleus dyeing, cytoplasm dyeing, dehydration, transparency, sealing and the like. Compared with an existing paraffin section making method, the making method has the advantages that fish ovarian tissue paraffin preparation and fixation effects are greatly improved, the dyeing effect of fish ovarian tissue paraffin sections in series is improved, dehydration and transparency effects are perfected, completeness and definition of fish ovarian tissue structures under the microscope are greatly enhanced, and a plurality of problems of a fish ovarian tissue paraffin section making process in the prior art are solved. Supporting conditions are provided for research of applications in the fields of pathomorphism, immunology, cell in-situ nucleic acidhybridization and morphometry, and further fish ovarian related research from gene and protein level.

Description

technical field [0001] The invention belongs to the technical field of making paraffin slices of biological tissues, and in particular relates to a method for making paraffin slices of fish ovarian tissues. Background technique [0002] Due to its simple operation and low price, paraffin section has always been the most widely used method for making sections in histological microscopic observation. As a traditional biological research technique, paraffin section can not only observe the morphology of normal tissue cells, but also has a wide range of applications in the fields of pathology, immunology, in situ nucleic acid molecular hybridization, and morphometrics. Paraffin sections should go through the steps of fixation, dehydration, transparency, paraffinization, embedding, sectioning, patching, dewaxing, staining, transparency, and mounting to keep the original structure of tissue cells and allow them to be clearly observed and analyzed. However, fish ovaries are rich i...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 陈剑杰曹谨玲罗永巨高荣琨
Owner SHANXI AGRI UNIV
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