Machilus cicatricosa tissue culture rapid propagation method

Abstract

The invention discloses a machilus cicatricosa tissue culture rapid propagation method. The machilus cicatricosa tissue culture rapid propagation method includes the following steps: (1) obtaining ofsterile seedlings, to be more specific, machilus cicatricosa seeds are wrapped in gauze, rinsed first with water, soaked in an alcohol solution, rinsed with sterile water, sterilized, and rinsed withthe sterile water, and residual water on the surface of the seeds is absorbed by sterile filter paper, finally the seeds are inoculated into a culture flask containing a minimal medium for culturing to obtain explants multiple shoots; (2) proliferation culture, to be more specific, the explants multiple shoots obtained in the step (1) are inoculated in a proliferation medium for culturing and subculture once every 60 days to obtain seedlings; and (3) rooting culture, to be more specific, the seedlings obtained in the step (2) are cut into sections and inoculated in a rooting medium for rootingculture. The method has a short culture period and is not affected by seasons, and through seedling hardening and transplanting, the survival rate of the seedlings is increased, the yield of the machilus cicatricosa is high, and the large-scale application of the machilus cicatricosa is promoted.

Description

technical field [0001] The invention belongs to the technical field of tree propagation, and in particular relates to a method for tissue culture and rapid propagation of Phoebe chinensis. Background technique [0002] Machilus cicatricosa is an evergreen tree of the genus Machilus Nees in the family Lauraceae, with a height of 15-20m. The bark is gray-brown with obvious scars after the scales fall off, the branchlets are dark brown; the leaves are oval, thin leathery, and the back of the leaves is pinkish green, clustered near the tip of the branchlets; the lateral veins are slightly slender, each side 12- 14; cymes terminal, fruit dark green, oblong; flowering period: May-June; fruit period: July-October, is an excellent native tree species for landscaping. But in fact, under conventional breeding conditions, the seedlings have great variation, and the residual growth speed is uneven. Tissue culture breeding technology has the advantages of high reproduction coefficient,...

AI Technical Summary

Problems solved by technology

But in fact, under conventional breeding conditions, the seedlings have great variation, and the growth rate residuals are not uniform.

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