Glomerulus system based on micro-fluidic chip and applications of glomerulus system

A technology of microfluidic chip and glomerulus, applied in the field of glomerular system, can solve the problem of inability to reproduce renal filtration function in vitro, and achieve the effect of reducing the failure rate

Active Publication Date: 2018-04-24
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

There have been a lot of work related to the simple in vitro culture of kidney cells for evaluation, most of which focus on simple cytotoxicity, and the...

Method used

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  • Glomerulus system based on micro-fluidic chip and applications of glomerulus system
  • Glomerulus system based on micro-fluidic chip and applications of glomerulus system
  • Glomerulus system based on micro-fluidic chip and applications of glomerulus system

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Experimental program
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Effect test

Embodiment 1

[0031] Construction and functional characterization of glomerular chips

[0032] A microfluidic chip, such as figure 2 Shown: The microfluidic chip consists of the left main channel 1, the right main channel 4, the collagen channel 3, the collagen channel inlet 5, the left main channel inlet 6 and the right main channel inlet 7. The collagen channel 3 is connected to the left Left main channel 1, right connected to right main channel 4.

[0033] The left main channel 1 is I-shaped, the right main channel 4 of the chip is U-shaped, and the collagen channel 3 is in the shape of "Feng", which is a horizontal structure in the middle of the collagen channel. The structure is connected with the left main channel 1 and the right main channel 4, and the number of transverse structures is 3; the junction of the collagen channel 3 and the left main channel 1 is a hemispherical interface, and the collagen side is concave.

[0034] The microfluidic chip is composed of two parts with di...

Embodiment 2

[0038] Evaluation of nephrotoxicity

[0039] A glomerular chip was constructed, and cell culture medium containing 0, 1, 2, 4, 8, and 16 μM of cadmium acetate was added for culture. After 24 hours of treatment, the culture medium in the main channel was collected for LDH leakage detection. The results are as follows: Figure 4 c shown. It can be seen that with the increase of the concentration of cadmium acetate, the leakage of LDH in the glomerulus increases, and the high concentration of cadmium acetate produces obvious nephrotoxicity. Quantitative test of cck-8 and live and dead staining of glomeruli were performed, and photos were taken by fluorescence microscope. The results are as follows Figure 5a and Figure 5 b shown. As the concentration of cadmium acetate increased, the number of dead cells in the glomerulus increased, and the cell viability decreased significantly. High concentrations of cadmium acetate produced significant nephrotoxicity. Igg (red fluorescenc...

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Abstract

The invention relates to a glomerulus system based on a micro-fluidic chip and applications of the glomerulus system. The micro-fluidic chip is composed of a left main channel, a right main channel, acollagen channel, a collagen channel inlet, a left main channel inlet and a right main channel inlet, wherein the left main channel is connected to the left side of the collagen channel, and the right main channel is connected to the right side of the collagen channel; the left main channel is inoculated with a glomerulus micro-tissue, the right main channel is used as a collecting area, the filtering effect of the glomeruli can be observed, and the influences of the renal toxicity caused by the chemical ingredients on the filtering function of the glomeruli also can be observed. According tothe technical scheme, the construction of a glomerulus chip with the filtering function outside the body is realized, and the application in the kidney toxicity evaluation of the medicines is also realized.

Description

technical field [0001] The invention relates to the technical field of applying microfluidic chip technology to the construction of in vitro organ models, in particular to a glomerular system based on a microfluidic chip and its application. Background technique [0002] Kidney is one of the main target organs of drug toxicity. The kidney is composed of more than 20 kinds of cells with different ultrastructure, metabolic capacity and transport function, and is an important organ for drug excretion. When chemical drugs are excreted by the kidneys, they will be selectively enriched in renal cells, causing damage to cell membranes, mitochondria, endoplasmic reticulum and lysosomes, destroying cell integrity, and causing renal cell apoptosis or necrosis. Reduce glomerular filtration and renal tubular reabsorption, resulting in an imbalance of water and electrolytes in the body, and even acute renal failure in severe cases. Therefore, observation of drug nephrotoxicity is an im...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/071C12Q1/02
CPCC12M21/08C12M23/16C12N5/0684C12N2503/02C12N2533/54G01N33/5014G01N2500/10
Inventor 秦建华李中玉陶婷婷郭雅琼
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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