Method for detecting catecholamine and metabolites by using liquid chromatography-tandem mass spectrometry technology

A technology for catecholamines and metabolites, which is applied in the field of liquid chromatography tandem mass spectrometry to detect catecholamines and metabolites, and can solve the problems of prolonged detection time, inhibition of application and the like

Inactive Publication Date: 2018-04-27
GUANGDONG ZHONGKEKANGYI BIOTECH CO LTD
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  • Abstract
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Problems solved by technology

This leads to an excessive extension of the detect...

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  • Method for detecting catecholamine and metabolites by using liquid chromatography-tandem mass spectrometry technology
  • Method for detecting catecholamine and metabolites by using liquid chromatography-tandem mass spectrometry technology
  • Method for detecting catecholamine and metabolites by using liquid chromatography-tandem mass spectrometry technology

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Embodiment Construction

[0026] The present invention discloses a method for detecting catecholamines and metabolites by liquid chromatography tandem mass spectrometry, comprising the following steps:

[0027] (1) Mix 250 μL ammonium acetate solution, 100 μL internal standard solution of catecholamines and their metabolites, and 200 μL plasma samples thoroughly before use;

[0028] (2) Mix N 200 μL standard solutions of catecholamines and their metabolites with different concentrations, respectively, with 250 μL of ammonium acetate solution and 100 μL of internal standard solutions of catecholamines and their metabolites to form N standard mixed solutions, ready to use ; wherein N is an integer of 4 to 10, preferably an integer of 5 to 8.

[0029] (3) Pretreat the weak cation exchange 96-well solid phase extraction plate with 200 μL of methanol and water respectively; Wash the sample wells with ammonium acetate solution and a mixture of acetonitrile and isopropanol, then use two 50 μL portions of for...

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Abstract

The invention discloses a method for detecting catecholamine and metabolites by using liquid chromatography-tandem mass spectrometry technology. The method can be used for one-time detection of catecholamine and metabolites thereof with high sensitivity and specificity. The method can be used for detecting MN and NMN (indices which are firstly recommended in an association guide), E and NE (effects for auxiliary diagnosis) as well as DA and 3-MT, and the method can be used for carrying out diagnosis of PPGL of various types with very high sensitivity and specificity, wherein the sensitivity and the specificity respectively reach 100% and 97.2%.

Description

technical field [0001] The invention relates to the technology in the field of compound detection, in particular to a method for detecting catecholamines and metabolites by liquid chromatography tandem mass spectrometry. Background technique [0002] Pheochromocytoma and paraganglioma (PPGL) are tumors that arise from the chromaffin cells of the adrenal medulla or the extra-adrenal sympathetic ganglion cells, respectively. PPGL will secrete a large amount of catecholamines, including epinephrine (E), norepinephrine (NE) and dopamine (DA), causing the patient's blood pressure to rise paroxysmal or persistently. Although PPGL patients only account for 0.2% to 0.6% of general hypertensive patients, if these patients are not treated in time, long-term hypertension can cause serious damage to the heart, brain, kidney and other organs of the patients. Some patients even develop hypertensive crisis, which is life-threatening. And if the patient can get an accurate and timely diag...

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Application Information

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IPC IPC(8): G01N30/96G01N30/72
Inventor 汪纯杨绍杰
Owner GUANGDONG ZHONGKEKANGYI BIOTECH CO LTD
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