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Method for domesticating sulfate-resistant type anaerobic ammonium oxidation bacteria

A kind of anammox bacteria and anaerobic ammonium oxidation technology, applied in chemical instruments and methods, methods using microorganisms, food industry wastewater treatment, etc., can solve the problems of long domestication period and poor sludge stability, and achieve low ammonia nitrogen Concentration, efficient denitrification, enhanced activity and adaptability

Inactive Publication Date: 2018-05-04
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it has the disadvantages of long domestication period and poor sludge stability.

Method used

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  • Method for domesticating sulfate-resistant type anaerobic ammonium oxidation bacteria
  • Method for domesticating sulfate-resistant type anaerobic ammonium oxidation bacteria
  • Method for domesticating sulfate-resistant type anaerobic ammonium oxidation bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The red granular anammox sludge was used as seed sludge for domestication. The inoculum amount of the sludge is 3.5L, and the MLSS of the sludge is 9000mg / L. The activated sludge is added to the reactor from the top of the reactor, and the reactor is sealed. The artificially simulated wastewater was used to start the experiment, and the concentration of ammonium sulfate was 1.47g / L (NH 4 + -N concentration is 200mg / L, SO 4 2-Concentration is 1067mg / L). Sodium nitrite artificial wastewater mainly provides nitrogen, trace elements and macroelements necessary for microbial growth. The pH of the influent water is adjusted at 7.5, the water temperature is adjusted to 31±1°C, and the concentration of sodium bicarbonate is controlled at 1500mg / L. High-purity nitrogen gas needs to be introduced into the bucket for storing artificial wastewater to completely dissolve the agent and blow out the dissolved oxygen.

[0034] The reactor uses a UASB reactor with reflux and thre...

Embodiment 2

[0037] Except that the dosage of sodium sulfate is increased to 3g / L each time, other conditions are kept the same as in Example 1 until the sulfate radical concentration in the influent is 18000mg / L. The total nitrogen removal rate, Heme c content and 16s rRNA copy number were measured when sodium sulfate was stopped.

Embodiment 3

[0039] Except that the dosage of sodium sulfate is increased by 5g / L each time, other conditions are kept the same as in Example 1 until the sulfate radical concentration in the influent is 18000mg / L. The total nitrogen removal rate, Heme c content and 16s rRNA copy number were measured when sodium sulfate was stopped.

[0040] When the addition of sodium sulfate was stopped, see Table 1 for the comparison of the total nitrogen removal rate, Heme c content and 16s rRNA content of the three implementation cases.

[0041] Table 1 When the addition of sodium sulfate is stopped, the total nitrogen removal rate, Heme c content and 16s rRNA content comparison of the three implementation cases

[0042]

[0043] In the three examples, from Example 1 to Example 3, the total nitrogen removal rate increases gradually, from 53.1% to 78.2%, which is an increase of 47.18%, which proves that the effect of the domestication method described in this patent is relatively good. The content o...

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Abstract

The invention provides a method for domesticating sulfate-resistant type anaerobic ammonium oxidation bacteria by manually configuring wastewater under a proper condition. The method disclosed by themethod adopts a manner of increasing the sulfate concentration of the artificial wastewater step by step, so that the anaerobic ammonium oxidation bacteria adapt to the high-sulfate environment and the output water reaches the standard. Each sulfate concentration domestication cycle is 4 days, domestication adopts an upflow anaerobic sludge bed (UASB) reactor, and the flora cultivated by the method are suitable for treating high-sulfate high-ammonia-nitrogen wastewater. The method has the following beneficial effects: the growth and reproduction requirements of the anaerobic ammonium oxidationbacteria are met completely, the anaerobic ammonium oxidation bacteria can gradually adapt to the sulfate radical environment with certain concentration within short time, the cultivated culture cantreat the wastewater containing high-concentration sulfate radical (such as monosodium glutamate wastewater) and is suitable for large-scale cultivation and commercialized application, the activity and the adaptability of the culture are enhanced, and good environment and economic effects are achieved.

Description

technical field [0001] The invention relates to a method for cultivating sulfate-resistant anammox bacteria, which belongs to the technical field of microbial cultivation, and in particular to a highly sulfate-tolerant anammox strain and a preparation and domestication method thereof, which can be specifically applied It is used to treat monosodium glutamate production wastewater and other wastewater containing high concentrations of sulfate and high ammonia nitrogen. Background technique [0002] Anammox bacteria are autotrophic bacteria that can use ammonia as an electron donor and nitrite as an electron acceptor to produce nitrogen under anoxic conditions. Anaerobic ammonium oxidation reaction, as a high-efficiency, single strong denitrification reaction, has been applied in the treatment of nitrogen-containing wastewater in a small range. Its reaction is easily affected by external conditions. It is necessary to increase its reaction rate and avoid the influence of exter...

Claims

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Application Information

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IPC IPC(8): C12N1/36C02F3/34C02F101/16C02F101/10C02F103/32
CPCC02F3/34C02F2101/101C02F2101/16C02F2103/32C12N1/36
Inventor 臧立华姬丹丹毛家明
Owner QILU UNIV OF TECH
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