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Mink venous blood collection method

A mink and vein technology, applied in the fields of medical science, sensors, diagnostic recording/measurement, etc., can solve the problems of inability to continuously collect blood for multiple times, difficult to capture and fix, easy to injure and other organs, etc., to improve blood collection efficiency and blood. The quality, the blood collection method is simple, and the effect is beneficial to the application

Inactive Publication Date: 2018-05-08
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the fact that the mink is covered with fur, small in size and flexible in movement, it is difficult to capture and fix it, and it is even more difficult to collect blood. In the scientific research and experiment of mink, blood collection is required many times. In clinical practice, toenail clipping or heart blood collection are often used. Both methods have many disadvantages. Toenail clipping and blood collection cannot guarantee sterility. Cardiac blood collection is more harmful to the heart, and it is also easy to injure other organs, causing internal bleeding and affecting normal growth. Usually, the needle punctures the heart and liver and causes bleeding. die of too much
Moreover, neither of the two methods can collect blood for multiple times in a row. In this way, many researchers have locked the blood collection object in the vein of the mink. Considering that a large amount of blood is needed for scientific research, ordinary small veins cannot meet this requirement. Requirements, need to find a vein that can provide sufficient blood and convenient and easy blood collection method to replace toenail clipping and heart blood collection

Method used

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  • Mink venous blood collection method
  • Mink venous blood collection method
  • Mink venous blood collection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method of mink vein blood collection is as follows:

[0034] 1) The animal is a mink, and the materials and utensils used in the blood collection method include: mink catching gloves, blood collection needles from the large anterior cavity, disinfectant alcohol, cotton balls, vacuum tubes, and cages for mink suits.

[0035] 2) The blood collection needle of the large anterior cavity vein is connected to a soft silicone tube at the rear end. The blood collection method includes mink feeding before blood collection, blood collection preparation, and blood collection steps. The process is as follows: the minks are adaptively raised for 2-3 days, and then blood collection is performed: the experimenter’s Wear a mink catching glove with one hand, and beat the mink cage with the other hand to drive the mink to the designated place. Use the gloved hand to gently catch the mink, place it prone, and inject anesthetics into the buttocks at a dose of 10 mg / kg body weight. When...

Embodiment 2

[0037] The method of mink vein blood collection is as follows:

[0038]1) The animal is a mink, and the materials and tools used in the blood collection method include: mink catching gloves, a large vein blood collection needle in the anterior cavity, sterile alcohol, cotton balls, vacuum tubes, and a cage for mink suits, and the blood collection needle is a disposable venous blood sample collection needle , consisting of a rubber sleeve, a needle tube at the docking end, a needle seat, a hose, a needle handle, a needle tube at the venipuncture end, and a protective sleeve at the venipuncture end; the negative pressure blood sample collection tube is composed of a negative pressure storage vessel, a rubber plug and a plastic cap; After finding the anterior vena cava, insert the needle tube at the venipuncture end into the negative pressure storage vessel. The specific structure of the blood collection needle and the blood collection tube is as follows: Figure 4-5 shown;

[0...

Embodiment 3

[0041] The method of mink vein blood collection is as follows:

[0042] 1) The animal is a mink, and the materials and tools used in the blood collection method include: mink catching gloves, a large vein blood collection needle in the anterior cavity, sterile alcohol, cotton balls, vacuum tubes, and a cage for mink suits, and the blood collection needle is a disposable venous blood sample collection needle , consisting of a rubber sleeve, a needle tube at the docking end, a needle seat, a hose, a needle handle, a needle tube at the venipuncture end, and a protective sleeve at the venipuncture end; the negative pressure blood sample collection tube is composed of a negative pressure storage vessel, a rubber plug and a plastic cap; After finding the anterior vena cava, insert the venipuncture needle into the negative pressure storage vessel;

[0043] 2) The blood collection needle of the large anterior cavity vein is connected to a soft silicone tube at the rear end. The blood ...

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Abstract

The invention provides a mink venous blood collection method which includes the steps: fixing a mink, disinfecting a manubrium and the periphery of the manubrium of the mink, taking the tail end of the manubrium as a datum point, forming an angle of 30-45 degrees between the manubrium and a sternal bar and taking the right centripetal position of a body median line as a blood collection site; stabbing the blood collection site by a blood collection needle, and enabling blood to flow into the blood collection needle if stabbing an anterior vena cava after repeated needling tests; connecting a blood collection tube and collecting the blood. According to the mink venous blood collection method, the blood is collected at the position of the anterior vena cava of the mink, a traditional toenailcutting and heart blood collection method is replaced, irritation and injury to the mink caused by the blood collection process are reduced, the requirement of animal welfare is met, blood collectionefficiency and blood quality are improved, application of a blood sample in an animal experiment research process is more facilitated, the experimental requirement of the blood sample is met, and theblood sample can be more effectively applied to the field of physiological and biochemical research.

Description

technical field [0001] The invention relates to the technical field of blood collection, in particular to a method for collecting mink vein blood. Background technique [0002] The body of mink is slender, the male body length is 38-42 cm, the tail length is 20 cm, and the body weight is 1.6-2.2 kg, while the female is smaller. The body hair is yellowish brown, with white spots on the jaws, the head is small, the eyes are round, and the ears are semicircular, slightly higher than the head and inclined to the front, and cannot swing. The neck is thick and short, the limbs are thick, the forelimbs are slightly shorter than the hind limbs, there are webs between the fingers and toes, the webs between the rear toes are more obvious, and there are fleshy pads on the soles of the feet. The tail is slender and the hair is fluffy. Mainly nocturnal and eats small rodents, but also mole crickets, frogs and fish. [0003] In view of the fact that the mink is covered with fur, small ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/153
CPCA61B5/15003A61B5/150061A61B5/153A61B2503/40
Inventor 陈杰赵建军闫喜军罗国良王小龙
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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