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Preparation method and application of medical porous Al(OH)3 adjuvant

An adjuvant, double-distilled water technology, applied in the field of medical materials and chemistry, can solve the problems of difficulty in determining the exact dose, affecting the quality and reproducibility of animal models of allergic diseases, and not having the prospect of clinical use, and achieving stable adsorption. effect of effect

Inactive Publication Date: 2018-05-08
周柳青
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, commercially available Al(OH) 3 Adjuvants do not have the exact adsorption capacity for specific antigens, such as: OVA (ovalbumin), or lack of determination of OVA adsorption capacity, making it difficult to determine the exact dosage in use, thus seriously affecting the quality of animal models of allergic diseases and repeatability
In addition, since the commercially available Al(OH) 3 The adjuvant lacks the necessary sterilization process during preparation, so it does not have the prospect of clinical use

Method used

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  • Preparation method and application of medical porous Al(OH)3 adjuvant
  • Preparation method and application of medical porous Al(OH)3 adjuvant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Porous Al(OH) 3 Adjuvant preparation

[0028] Anhydrous Al 2 (SO 4 ) 3 Dissolve in 100mL double-distilled water, slowly add 25% NH 3 ·H 2 O, control the dripping time for not less than 6.5 hours, during which the temperature of the reaction system should not exceed 40°C. Waiting for NH 3 ·H 2 After all the O is added dropwise, stir at room temperature for 24h, and then stand and age for 12h to obtain white Al(OH) 3 Sol. The resulting reaction product was repeatedly washed with double distilled water until there was no SO 4 2- can be detected. Then wash 3 times with 50 mL of absolute ethanol, each washing time is 10 min. Centrifuge, discard the supernatant, and store at room temperature with P 2 o 5 Vacuum dry for 48 hours as a desiccant to obtain white solid Al(OH) 3 . Then the obtained Al(OH) 3 The solid was put into an ethylene oxide sterilizer and sterilized for 4 hours. White solid Al(OH) after sterilization 3 Soak in 50mL spectroscopica...

Embodiment 2

[0029] Example 2 Porous Al(OH) 3 Scanning electron microscope (SEM) image of adjuvant

[0030] Such as figure 1 Shown: Al(OH) prepared by the above method 3 Under the field emission scanning electron microscope of the adjuvant, it can be seen that dense holes with different calibers exist. These holes show that a kind of Al(OH) with porous structure prepared by the present invention 3 adjuvant. At the same time, the porous structure provides sufficient space for accommodating specific antigens.

Embodiment 3

[0031] Example 3Al(OH) 3 Determination of the adsorption amount of adjuvant to specific antigen OVA

[0032] According to the method reported in the literature, the Al(OH) 3 Adjuvant adsorption to specific antigen OVA at body temperature. The result is as figure 2 Shown: The maximum adsorption capacity is 130mg / g.

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Abstract

The invention discloses a preparation method and application of a medical porous Al(OH)3 adjuvant. The preparation method comprises the following steps: dissolving anhydrous Al(OH)3 into double distilled water; mechanically stirring at a high speed and slowly dropwise adding NH3.H2O; after dropwise adding all the NH3.H2O, stirring at room temperature; then standing and ageing to obtain white Al(OH)3 sol; repeatedly washing a reaction product with the double distilled water until no SO4<2-> is detected; then washing with absolute ethyl alcohol; centrifuging and separating; drying at room temperature and in vacuum by taking P2O5 as a drying agent, so as to obtain white solid Al(OH)3; then sterilizing by utilizing ethylene oxide; immersing the sterilized Al(OH)3 with spectroscopically pure acetone for certain days; changing the spectroscopically pure acetone 4 times every day; then filtering and drying in vacuum; sterilizing again by utilizing ultraviolet light to obtain the dry powder sterile Al(OH)3 adjuvant. The adjuvant disclosed by the invention has a remarkable and stable adsorption effect on common allergen VOA and a clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of medical materials and chemistry, and relates to a preparation method and application of a porous Al(OH)3 adjuvant which can be used in clinical medicine. Background technique [0002] Al(OH) 3 Adjuvant is the preferred adjuvant for establishing allergy animal models at home and abroad, and it is also the only human vaccine adjuvant approved by FDA so far. Relevant studies have confirmed that the effect of aluminum adjuvant on enhancing immunity depends on the adsorption of antigen by the adjuvant. The higher the amount of adsorbed antigen, the stronger the immune effect; conversely, the weaker the immune effect. Therefore, the ability to adsorb antigen is Al(OH) 3 Adjuvant is a key factor in enhancing the immune effect and an important objective standard for evaluating its adjuvant performance. [0003] Al(OH) 3 The adsorption performance of adjuvant to antigen is limited by its own microstructure, an...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/35A61P37/04A61P37/08A61L2/20A61L2/10A61L101/44
CPCA61K39/35A61K39/39A61K2039/55505A61L2/0047A61L2/0094A61L2202/21A61K2300/00
Inventor 周柳青
Owner 周柳青
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