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A processing method for snus samples and a detection method for aldehydes in snus

A detection method, snus technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome processing, reduced detection accuracy, and unfavorable rapid detection

Active Publication Date: 2020-10-20
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is cumbersome for the treatment process before UPLC analysis of smokeless tobacco, which is not conducive to rapid detection, and it is easy to cause the loss of detected substances and reduce the accuracy of detection.

Method used

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  • A processing method for snus samples and a detection method for aldehydes in snus
  • A processing method for snus samples and a detection method for aldehydes in snus
  • A processing method for snus samples and a detection method for aldehydes in snus

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Experimental program
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Effect test

Embodiment 1

[0053] The detection method of aldehydes in snus in this embodiment is to analyze and detect formaldehyde, acetaldehyde and crotonaldehyde in snus, including the following steps:

[0054] 1) Preparation of various solutions

[0055] 1.1) Preparation of derivatization reagent solution

[0056] Weigh 0.3g DNPH, dissolve it with acetonitrile and dilute to 100mL to obtain a derivatization reagent solution; store it at room temperature in the dark, and the validity period is 3 months;

[0057] 1.2) Preparation of ammonium formate buffer solution

[0058] Weigh 6.3g of ammonium formate, dissolve it in about 900mL of deionized water, add formic acid to adjust the pH to 3.0, and then dilute to 1L with deionized water to obtain ammonium formate buffer solution; store at room temperature, and the validity period is 1 month;

[0059] 1.3) The preparation of a series of standard working solutions includes the following steps:

[0060] 1.3.1) Preparation of standard stock solution

[0...

Embodiment 2

[0082] This embodiment is for the detection of formaldehyde, acetaldehyde and crotonaldehyde in bag-type snus; the detection method of aldehydes in snus, except that the weighing of snus samples is carried out according to the following steps, the remaining steps Completely with embodiment 1:

[0083] Take out the sealed packaging samples stored at -20°C, take out a small bag of snus (the mass of a small bag of snus is about 1g) and place it at 4°C for 2 days, and then equilibrate at room temperature Weigh after 2 hours, cut it open after weighing, put the tobacco powder together with the packaging bag into a 50mL Erlenmeyer flask; the remaining samples are sealed and packaged again and placed in a low temperature environment of -20°C.

Embodiment 3

[0085] The detection method of aldehydes in snus in this embodiment is to analyze and detect formaldehyde, acetaldehyde and crotonaldehyde in snus, including the following steps:

[0086] 1) Preparation of various solutions

[0087] 1.1) Preparation of derivatization reagent solution

[0088] Weigh 0.1g DNPH, dissolve it with acetonitrile and dilute to 100mL to obtain a derivatization reagent solution; store it at room temperature in the dark, and the validity period is 3 months;

[0089] 1.2) Preparation of ammonium formate buffer solution

[0090] Weigh 0.63g of ammonium formate, dissolve it in about 900mL of deionized water, add formic acid to adjust the pH to 2.5, and then dilute to 1L with deionized water to obtain ammonium formate buffer solution; store at room temperature, and the validity period is 1 month;

[0091] 1.3) The preparation of a series of standard working solutions includes the following steps:

[0092] 1.3.1) Preparation of standard stock solution

[...

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Abstract

The invention relates to a method for treating buccal tobacco sample and a method for detecting aldehyde substances of buccal tobacco, and belongs to the technical field of smoke-free tobacco productchromatographic analysis and detection. The method for treating the buccal tobacco sample comprises the following steps: adding an acidity pH regulator, a derivatization reagent and an extracting agent into the buccal tobacco sample sequentially and mixing uniformly, leaving to stand and layering, and taking the upper layer of organic phase as a to-be-detected sample, wherein the volume of the extracting agent corresponding to 1 g of buccal tobacco is 8 to 15 ml. According to the method for treating the buccal tobacco sample, leaching of the aldehyde substances of the buccal tobacco can be accelerated, the reaction time of the aldehyde substances and the derivatization reagent of the buccal tobacco is shortened, the product after reaction is extracted to enter the organic phase, the analysis and detection time is further shortened, the loss of the aldehyde substances in the analysis and detection process is reduced and the accurate degree of analysis and detection is increased.

Description

technical field [0001] The invention relates to a processing method for snus samples and a detection method for aldehyde substances in snus, belonging to the technical field of chromatographic analysis and detection of smokeless tobacco products. Background technique [0002] Smokeless tobacco products are a kind of tobacco products that consume tobacco through non-combustion methods. There are many types, and the mainstream product on the market is snus. Snus refers to the form of smokeless tobacco products that are consumed by snuffing. There are two main types, namely Swedish snus (Snus) and American snuff (Moist snuff). Swedish tobacco is a kind of wet tobacco powder with Swedish style, which is usually made of tobacco powder with a certain particle size mixed with an appropriate amount of flavoring substances, flavoring agents, water, humectants and acid-base regulators through heat treatment. The moisture content is generally between 30% and 60%, and there are two typ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 王丁众张启东李鹏刘珊马明范武柴国璧席辉孙世豪宗永立张建勋
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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