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Method for detecting extract product for reduction of blood sugar

A detection method and extract technology, applied in the field of biomedicine, can solve problems such as no blood sugar-lowering extract, no component detection method report, etc., and achieve the effect of remarkable curative effect.

Inactive Publication Date: 2018-05-15
JINAN HAOYU QINGTIAN PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At home and abroad, there is no drug that can completely cure diabetes. So far, there have been no reports or patents related to the hypoglycemic extracts from Neem leaves, leaves and Toona sinensis leaves. Conditional multi-component detection method report

Method used

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  • Method for detecting extract product for reduction of blood sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Preparation of hypoglycemic extract

[0036] (1) Take 1 part by weight of neem leaves, 1 part by weight of small yellow skin leaves, and 1 part by weight of Chinese toon leaves, mix them evenly, crush them, pass through a 20-mesh sieve, use cyclohexane as a solvent, and extract 3 times at 60 ° C. The extraction time is 2.5 hours each time, and the amount of solvent used each time is 12 times of the total weight of neem leaves, leaves and Chinese toon leaves, filtered, combined cyclohexane extracts, reclaimed cyclohexane under reduced pressure, concentrated and dried to obtain Crude extract A, the dregs extracted by cyclohexane for subsequent use;

[0037] (2) Extract the dregs after cyclohexane extraction in step (1) with ethyl formate-methanol mixed solvent with a volume ratio of 1:5, and extract 4 times at 75°C, each extraction time is 3.5 hours, each time the solvent The dosage is 15 times of the total weight of neem leaves, leaves and Chinese toon lea...

Embodiment 2

[0043] Embodiment 2: Preparation of hypoglycemic extract

[0044] (1) Take 3 kg of neem leaves, 2 kg of small yellow skin leaves, and 3 kg of Chinese toon leaves, mix them evenly, crush them, pass through a 20-mesh sieve, use cyclohexane as a solvent, and extract 3 times at 60 ° C, each extraction time is 2.5 hours , each solvent dosage is 12 times of the total weight of neem leaves, leaves and Chinese toon leaves, filters, merges the cyclohexane extract, reclaims the cyclohexane under reduced pressure, concentrates and dries to obtain the crude extract A, and The medicinal residue after hexane extraction is standby;

[0045] (2) Extract the dregs after cyclohexane extraction in step (1) with ethyl formate-methanol mixed solvent with a volume ratio of 1:5, and extract 4 times at 75°C, each extraction time is 3.5 hours, each time the solvent The dosage is 15 times of the total weight of neem leaves, leaves and Chinese toon leaves, filter, combine the ethyl formate-methanol ext...

Embodiment 3

[0051] Embodiment 3: Preparation of hypoglycemic extract

[0052] (1) Take 2 kg of neem leaves, 2 kg of small yellow skin leaves, and 5 kg of Chinese toon leaves, mix well, crush them, pass through a 20-mesh sieve, use cyclohexane as a solvent, and extract 3 times at 60 ° C, each extraction time is 2.5 hours , each solvent dosage is 12 times of the total weight of neem leaves, leaves and Chinese toon leaves, filters, merges the cyclohexane extract, reclaims the cyclohexane under reduced pressure, concentrates and dries to obtain the crude extract A, and The medicinal residue after hexane extraction is standby;

[0053] (2) Extract the dregs after cyclohexane extraction in step (1) with ethyl formate-methanol mixed solvent with a volume ratio of 1:5, and extract 4 times at 75°C, each extraction time is 3.5 hours, each time the solvent The dosage is 15 times of the total weight of neem leaves, leaves and Chinese toon leaves, filter, combine the ethyl formate-methanol extract, r...

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Abstract

A technical scheme discloses a method for detecting an extract product for reduction of blood sugar. The extract product for reduction of blood sugar is prepared by the following steps: leaves of Chukrasia tabularis, leaves of Clausena emarginata, and leaves of Toona sinensis are used as raw materials; extraction, separation and purification are carried out in order to obtain the extract product for reduction of blood sugar containing clauemargine B, libiguin A, teuvisside A, and aphanamixoid F. The method for detecting employs a silica gel G thin layer plate for detection, four characteristicspots are visible, HPLC is used for detection, and four characteristic chromatographic peaks are visible.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a detection method of a hypoglycemic extract, in particular to a detection method of a hypoglycemic extract prepared from neem leaves, pectinosa leaves and Chinese toon leaves as raw materials. Background technique [0002] Diabetes mellitus is a metabolic disease caused by insufficient insulin secretion or insensitivity of peripheral tissues to insulin. etc.) long-term impairment, insufficiency or failure. According to different pathogenesis, diabetes can be divided into type Ⅰ diabetes, type Ⅱ diabetes, gestational diabetes and other types of diabetes. [0003] According to statistics, in 2015, about 415 million people aged 20-79 in the world suffered from diabetes (8.8% prevalence rate), and another 318 million people had impaired glucose tolerance (pre-prevalence rate was 6.7%). China is the country with the largest number of diabetic patients in the world. In 2015, the number of p...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/88A61K36/75A61P3/10
CPCA61K36/58A61K36/75A61K2236/331A61K2236/333A61K2236/35A61K2236/39A61K2236/55G01N30/88G01N30/90G01N2030/8809A61K2300/00
Inventor 不公告发明人
Owner JINAN HAOYU QINGTIAN PHARMA TECH CO LTD