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A technology for aflatoxin and tea, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of difficulty in widespread use, long detection time, and high detection cost, and achieves improved detection sensitivity, good purification effect, and high detection sensitivity. Effect
Pending Publication Date: 2018-05-18
云南健牛环境监测有限公司
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Problems solved by technology
Although the detection results of HPLC-MS / MS are reliable, the detection cost is high, the detection time is long, it is difficult to be widely used, and there is also some interference
No purification methods and materials that can be used to eliminate interference in detection have been reported so far
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Embodiment 1
[0029] Embodiment 1 utilizes the present invention to detect the content of aflatoxin B1 in Pu'er ripe tea
[0030] 1. Polydopamine and CTAB-coated Fe 3 o 4 Preparation of Nanomaterials
[0031] (1) Fe 3 o 4 Preparation of nanomaterials: Take 5.0 g of ferrous ammonium sulfate and 2.8 g of ferric chloride, dissolve them in 100 mL of purified water, and heat them in a water bath to 75 °C under nitrogen protection, then add 10 mL of ammonia water, continue stirring for 40 min, and finally Cool to room temperature, wash 3 times with 50 mL of pure water, and vacuum dry at 60°C for 48 hours to obtain Fe 3 o 4 nanomaterials;
[0032] (2) Polydopamine and CTAB-coated Fe 3 o 4 Preparation of nanomaterials: take the Fe prepared in step (2) 3 o 4 1 g of nanomaterials was dispersed in 30 mL of deionized water and 25 mL of 10 mM dopamine solution, the pH was adjusted to 8.5 with 0.1 M NaOH, 0.02 g of potassium persulfate was added, and stirred at room temperature for 2 h. Then add...
Embodiment 2
[0051] Embodiment 2 utilizes the present invention to detect the content of aflatoxin in black tea
[0052] 1. Polydopamine and CTAB-coated Fe 3 o 4 Preparation of Nanomaterials
[0053] (1) Fe 3 o 4 Preparation of nanomaterials: Take 4.1 g of ferrous ammonium sulfate and 4.0 g of ferric chloride, dissolve them in 100 mL of purified water, and heat them in a water bath to 85 °C under nitrogen protection, then add 10 mL of ammonia water, continue stirring for 30 min, and finally Cool to room temperature, wash 5 times with 50 mL of pure water, and vacuum dry at 70°C for 48 hours to obtain Fe 3 o 4 nanomaterials;
[0054] (2) Polydopamine and CTAB-coated Fe 3 o 4 Preparation of nanomaterials: take the Fe prepared in step (2) 3 o 4 2 g of nanomaterials were dispersed in 50 mL of deionized water and 35 mL of 10 mM dopamine solution, the pH was adjusted to 8.5 with 0.1 M NaOH, 0.05 g of potassium persulfate was added, and stirred at room temperature for 3 h. Then add 0.5 ...
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Abstract
The invention provides a method for detecting AFB1 (aflatoxin B1) in tea and aims at the problem that coexistent matter in Pu'er tea severely interferes with determination of AFB1. Two functional magnetic nanomaterials with effects of superficially adsorbing AFB1 and purifying the interference matter are prepared, during use, a mixture of the two materials is adopted to finish adsorption and purification simultaneously, AFB1 in Pu'er tea is determined through combination with HPLC (high-performance liquid chromatography)-fluorescence detector, and an accurate determination result is obtained;the established method is compared with HPLC-MS/MS (tandem mass spectrometry), and results are in agreement. According to the prepared functional magnetic Fe3O4 nanoparticles, strong adsorption of AFB1 by polydopamine, good dispersion of cationic surfactant CTAB and hydrophilic interaction of surfaces of CQDs prepared from dopamine are sufficiently used, so that the mixture of the two functional magnetic nanomaterials has adsorption and purification functions on AFB1 in Pu'er tea. The preparation materials are nanoscale, so that material consumption is low, only several milligrams are needed,the cost is low, the detection time is short and only several minutes are needed, and based on the characteristics, the method has huge advantages in determination of AFB1 in Pu'er tea.
Description
technical field [0001] The invention relates to a method for detecting aflatoxin B1 in tea. It belongs to the field of food testing. Background technique [0002] Aflatoxins (AFT) are a class of compounds with similar chemical structures, all of which are derivatives of dihydrofuranocoumarins. Aflatoxins are secondary metabolites mainly produced by Aspergillus flavus and Aspergillus parasiticus, and the probability of aflatoxins appearing in food and feed in hot and humid areas is the highest. AFT shows severe toxicity to humans and various animals, and has obvious carcinogenicity, among which aflatoxin B1 (AFB1) is the most toxic and carcinogenic. Therefore, if there is aflatoxin in food, it will greatly threaten the health of consumers. my country is a big country in tea production and sales, but currently there is no limit standard for aflatoxins in tea. In 2011, a survey found that the detection rate of aflatoxins in Pu-erh tea was relatively high, and tea became a n...
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