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Improved immunohistochemical repairing solution

A technology of immunohistochemistry and repair solution, applied in the field of immunology, can solve the problems of poor repair effect of immunohistochemical repair solution, affect staining and diagnosis, difficult antigen detection, etc., and achieve good staining effect, easy staining and repair effect Good results

Active Publication Date: 2018-05-18
珠海霍普金斯医药研究院股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many antigens are difficult to detect clinically, and the repair effect of conventional immunohistochemical repair solutions is not good, which directly affects subsequent staining and diagnosis

Method used

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  • Improved immunohistochemical repairing solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Each 1000mL modified immunohistochemical repair solution includes: 30g trisodium citrate, 0.75mL NP-40, 7.5mL Tween-20 and the rest double distilled water.

[0028] The preparation method is as follows: Weigh trisodium citrate and dissolve it with double distilled water; add NP-40, add Tween-20 after it is completely dissolved, and distill the volume to the rated capacity with double distilled water after Tween-20 is dissolved.

Embodiment 2

[0030] Each 1000mL modified immunohistochemical repair solution includes: 20g trisodium citrate, 4mL NP-40, 2mL glycerin and the rest double distilled water.

[0031] The preparation method is as follows: Weigh trisodium citrate and dissolve it with triple distilled water; add NP-40, add glycerin after it is completely dissolved, and dilute to the rated capacity with double distilled water after glycerin is dissolved.

Embodiment 3

[0033] Each 1000mL modified immunohistochemical repair solution includes: 40g trisodium citrate, 0.1mL NP-40, 20mL Tween-20 and the rest double distilled water.

[0034] Preparation method is the same as embodiment 1

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Abstract

The invention relates to an improved immunohistochemical repairing solution. The improved immunohisochemical repairing solution is prepared from trisodium citrate, NP-40 (Nonidet P 40) and an emulsifying agent. According to the improved immunohistochemical repairing solution disclosed by the invention, a repairing effect is better, and tissue slices can be repaired under less dosage of the improved immunohisochemical repairing solution; the improved immunohisochemical repairing solution is suitable for antigens which are relatively difficult to be detected clinically, such as Fas (Fatty Acid Synthase), Bax (Bcl-2 associated X protein), FVIII (Factor VIII), Lamnin, CoIV, Keratin, CEA (Carcino Embryonie Antigen) and GFAP (Glial Fibrillary Acidic Protein); the tissue slices are easy to be dyed after being repaired by adopting the improved immunohistochemical repairing solution, and a dyeing effect is better.

Description

technical field [0001] The invention relates to the field of immunology, in particular to an improved immunohistochemical repair solution. Background technique [0002] Antigen refers to a substance that can stimulate the body to produce a (specific) immune response, and can combine with immune response product antibodies and sensitized lymphocytes in vivo and in vitro to produce an immune effect (specific reaction). The basic properties of antigens are foreign body, macromolecules and specificity. Foreign body refers to the antigenic substance entering the body tissue, which must be different from the composition of the body tissue cells. Antigens generally refer to foreign substances that enter the body, such as bacteria, viruses, pollen, etc.; antigens can also be substances between different species, such as horse serum entering the body of rabbits, and many proteins in horse serum become rabbit antigens Allogeneic substances can also become antigens, such as blood typ...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N1/28
CPCG01N1/28G01N33/531
Inventor 刘天赫王重庆赵柏松
Owner 珠海霍普金斯医药研究院股份有限公司
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