Microfluidic singe cell gene expression detection chip as well as working method and preparation method thereof

Abstract

The invention provides a microfluidic singe cell gene expression detection chip as well as a working method and a preparation method thereof. The chip comprises a substrate layer, a passivation layer,a magnet layer, a flowing layer and a pneumatic control layer, wherein the passivation layer is arranged on the substrate layer; the magnet layer is arranged on the passivation layer; the flowing layer is arranged on the magnet layer; the pneumatic control layer is arranged on the flowing layer; the substrate layer is provided with an electrode; the pneumatic control layer is divided into a biochemical analysis unit array; the biochemical analysis unit array comprises a plurality of biochemical analysis units; each biochemical analysis unit is divided into a cell capturing region and a biochemical reaction region. The microfluidic singe cell gene expression detection chip can be used for effectively improving the precision, sensitivity, efficiency and the like of molecular detection; sample cross contamination, operation complexity and reagent consumption are reduced.

Description

technical field [0001] The invention belongs to the field of chips, and in particular relates to a microfluidic single-cell gene expression detection chip, a working method and a preparation method thereof. Background technique [0002] Micro / nano manufacturing mainly studies the scientific issues in the design and manufacture of functional structures, devices and systems with feature sizes in the range of microns and nanometers. Cells are the basic unit of life structure and function. Gene expression detection in living single cells is the limit state of cell life analysis technology, which is of great significance for revealing the mechanism of occurrence and evolution of major diseases and promoting the development of new drugs. Although microfluidic technology has supported major discoveries in basic biology and clinical medicine to a certain extent, and accelerated the progress of many hot topics in basic scientific research, existing chips have complex structures, poor...

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Problems solved by technology

Although microfluidic technology has supported major discoveries in basic biology and clinical medicine to a certain extent, and accelerated the progress of many hot topics in basic scientific research, existing chips have complex structures, poor integration, low detection throughput, and high cost. Insufficient problem

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