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Application of metabolite of physarum polycephalum used as biomarker

A technology of Phytophthora polycephalus and biomarkers, which is applied in the field of biochemistry and can solve the problems of limited detection methods of nTiO2

Pending Publication Date: 2018-05-22
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, provide a kind of application of the metabolite of Phymophora polycephalum as a biomarker, aim to solve the problem of nTiO in the existing environment 2 Technical issues with limited means of detection

Method used

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  • Application of metabolite of physarum polycephalum used as biomarker
  • Application of metabolite of physarum polycephalum used as biomarker
  • Application of metabolite of physarum polycephalum used as biomarker

Examples

Experimental program
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Effect test

Embodiment 1

[0058] TiO 2 Effects on the growth of Physarum polycephalum

[0059] In the absence of photocatalysis, nTiO 2Considered a biocompatible material. In this example, Physophora polycereus was exposed to increasing concentrations of nTiO-containing 2 (0mg / ml, 5mg / ml, 9mg / ml, 15mg / ml and 18mg / ml) in the solid culture medium three days, the weight of each group of Chrysomyces polycephalum is weighed; The result is as follows figure 1 Shown: Compared with the control group, the growth rate of Phymophora polycephalum was slightly increased (about 10% and 20%) in the concentrations of 5 mg / ml and 9 mg / ml; compared with the control group, higher concentrations of nTiO 2 (15mg / ml) significantly inhibited growth (about 50%), 18mg / ml of nTiO 2 Almost completely inhibits the growth of Physarum polycephalum.

Embodiment 2

[0061] TiO 2 Induction of Metabolic Disorders in Phymophthora polycephalum

[0062] Semiquantitative analysis of metabolite levels in Chrysophora polycephalum samples by GC / MS and multivariate statistical analysis including unsupervised principal component analysis (PCA) and supervised orthogonal partial least squares discriminant analysis (OPLS-DA) Evaluate the resolved peaks. PCA score plot ( figure 2 A) and OPLS-DA score chart ( figure 2 C) shows the control group and 9mg / ml nTiO 2 Significant differences in treatment groups. Each part of the OPLS-DA model explained the difference changes as X matrix (metabolite data) 61%, Y matrix (nTiO 2 Treatment group: 1, Control: 0) 99.7%, due to the cumulative Q of the prediction accuracy of the OPLS-DAY model matrix 2 was 91.6%, indicating a very significant difference between groups in multivariate analysis. Similarly, the PCA score plot ( figure 2 B) and OPLS-DA score map ( figure 2 D) shows the control and 15mg / ml nTi...

Embodiment 3

[0070] Physophora polycereus was nTiO 2 induced biochemical changes

[0071] High ROS levels can induce oxidative damage to biomacromolecules, so ROS levels can be used as indicators of oxidative stress. H can be used 2 DCFDA probe detects ROS levels to determine nTiO 2 Is it possible to increase ROS levels in Phymophora polycephalum under dark conditions. Such as Figure 5 Shown in A: the control group was exposed to nTiO after a brief exposure (100 min) whose ROS levels fluctuated within a relatively narrow range (0.8-1.2 times the initial value). 2 (9mg / ml and 15mg / ml), the ROS levels of Phymophora polycephalum increased rapidly after exposure, and reached the peak rapidly (2.2 times and 3 times, respectively). Although the ROS level decreased after reaching the peak, it was still higher than that of the control group, indicating that nTiO 2 Under dark conditions, an imbalance in the ROS levels of Phymophora polycephalum was induced.

[0072] 8-OHdG is one of the mai...

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Abstract

The invention belongs to the technical field of biochemistry, and concretely relates to application of a metabolite of physarum polycephalum used as a biomarker in detecting nanometer titania in a dark environment. The invention discusses the research of nTiO2 induce cell metabolism disturbance by adopting a slime mold as a model for the first time, meanwhile, provides a new perspective of an nTiO2 toxicity mechanism under a darkness condition, and finds out that the metabolite of the physarum polycephalum used as the biomarker can be used for detecting the nTiO2 in the dark environment; researches show that metabonomics can be used as a quick, reliable and powerful tool for researching interaction among organisms, the environment and a nanometer material.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to the application of a metabolite of Phymophora polycephalum as a biomarker. Background technique [0002] Nano titanium dioxide (nTiO 2 ) has been widely used in papermaking, coatings, textiles, cosmetics, animal feed and other fields. Nano-titanium dioxide is the second most important nano-material produced in China, with an annual output of 800-1800t, and the annual output of Europe and the United States is 55-3000t and 7800-38000t respectively. nTiO 2 Widespread commercial use and release into the environment requires an in-depth understanding of its toxicity mechanisms, when exposed to sunlight or UV light, nTiO 2 exhibited strong cytotoxicity. Studies have shown that when nTiO 2 After exposure to sunlight or UV radiation, abundant reactive oxygen species (ROS) such as O 2 · - and HO 2 , these ROS can cause oxidative damage to the cells of animals, pla...

Claims

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Application Information

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IPC IPC(8): C12Q1/02C12R1/645
CPCC12Q1/025G01N2333/37
Inventor 张智刘士德张建华张天任
Owner SHENZHEN UNIV
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