Use of albendazole in the preparation of medicines for treating glioblastoma
A glioblastoma, albendazole technology, applied in antitumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problem of high recurrence rate of glioblastoma, poor prognosis, difficult to completely remove glioblastoma tumor and other problems, to achieve the effect of inhibiting tumor cell growth, inhibiting tumor growth, and inhibiting vascular factors
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Embodiment 1
[0038] Example 1: Validation of the effect of drugs containing albendazole on inhibiting the growth and proliferation of glioblastoma
[0039] Glioblastoma stem cells were cultured from the U87 cell line (purchased from Beijing Biobowell Biotechnology Co., Ltd.). U87 cells were cultured in serum free medium (SFM) at a planting density of 100,000 cells / ml. After subculture, the cells that became non-adherent and showed a neurosphere-like growth pattern were used as GSC cells. The composition of SFM is: DMEM / F12 (1:1), HEPES buffer (10mM), EGF (20ng / ml; Invitrogen), bFGF (20ng / ml; Invitrogen), L-glutamine (2mM), glucose ( 0.3% w / v), N-2 Additive (R&D Systems).
[0040] Culture of Glioma Cell Lines U87, U251, A172
[0041] Human glioma cells U87, U251, and A172 were obtained from the Neurosurgery Department of Xijing Hospital, Fourth Military Medical University (purchased from Beijing Biobowell Biotechnology Co., Ltd.), all in the presence of 10% fetal bovine serum, 100 U / ml p...
Embodiment 2
[0046] Example 2: Detection of cell apoptosis by flow cytometry
[0047] Digest and collect the cells treated with albendazole at each concentration for 24 h, adjust the cell concentration to 1×10 7 , take 100 μL each; after washing twice with Phosphate buffered solution (PBS), discard the supernatant; add 100 μL of pre-cooled binding buffer to resuspend the cells, put them in an ice bath; add 5 μL AnnexinⅤ-FITC and 5 μL PI In the cell suspension, mix gently; place the test tube at 4°C in the dark for 10 minutes; add 490 μL of binding buffer to suspend the cells, detect with flow cytometry, and analyze the percentage of cell apoptosis after medication. Repeat 3 times for each group. Such as Figure 6 as shown, Figure 6-1 For the control group, Figure 6-2 For the test group, after albendazole with a concentration of 100ng / ml acts on the glioblastoma cell line U251 cells, the results of the cell cycle are analyzed by flow cytometry. Albendazole makes the cells in the G1 ph...
Embodiment 3
[0048] Example 3: Validation of the effect of drugs containing albendazole on inhibiting the growth of glioblastoma in vivo
[0049] Xenograft tumor model in nude mice
[0050] Glioma stem cell GSC and cell line U87 cell suspension 0.2mL, containing 5×10 6 The cell viability was determined to be over 90% by trypan blue staining. Injected subcutaneously in the right armpit of nude mice. Measure the maximum long diameter (a) and the maximum short diameter (b) of the tumor with a vernier caliper every 3 days, according to the formula V=ab 2 / 2 to calculate the tumor volume.
[0051] In vivo administration of xenograft tumor in nude mice
[0052] The dosage of albendazole was set at 50, 100 mg / kg. Divide 30 tumor model nude mice into 6 groups (15 each for GSC and U87 models), 5 for each group, half male and half male, respectively GSC tumor model group, GSC albendazole 50 mg / kg group, GSC albendazole 50mg / kg group, GSC albendazole Dazol 100 mg / kg group, U87 tumor model group...
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