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A rapid cultivation method of denitrification granular sludge

A granular sludge and cultivation method technology, applied in biological sludge treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve problems such as different requirements for dissolved oxygen and instability in the formation of micro-anoxic zones, Achieve good settling properties, good total nitrogen removal effect, and stable performance

Active Publication Date: 2020-09-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention overcomes the problem that denitrification microorganisms have different requirements for dissolved oxygen, and solves the problem of unstable micro-anoxic zone formation in denitrification granular sludge flocs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Cultivation of denitrification granular sludge

[0020](1) Take the activated sludge from a petrochemical plant and put it into the aerobic reactor according to the MLSS of 3000mg / L, and carry out the enrichment culture of the aerobic sludge under the condition of alternating high and low dissolved oxygen, so as to obtain the high concentration of COD Bacteria capable of denitrification in the environment. The ammonia nitrogen concentration in the culture solution is 150mg / L, and the COD concentration is 1500mg / L. As a culture cycle every day, add the culture medium at one time, first culture under the condition of DO: 4-5mg / L for 12h, then reduce the DO to 1.0mg / L and culture for 11h, settle for 30 minutes, and discharge the supernatant for 15 minutes , the next batch of culture was carried out 15 minutes after supplementing with fresh culture medium. After 10 days of cultivation, when the sludge concentration increased to 5000mg / L, it entered the next stag...

Embodiment 2

[0024] Example 2 Performance test of denitrification granular sludge

[0025] (1) Mechanical strength test

[0026] Put equal amounts of the granular sludge obtained in Example 1 and the granular sludge obtained in CN102040281A into conical flasks containing 150mL of distilled water, and vibrate on a constant temperature shaker at 20°C and 150r / min for 24h to observe the degree of damage of the immobilized cells . The results showed that the granular sludge obtained in CN102040281A was slightly damaged during shaking, and after shaking for 24 hours, the turbidity of the supernatant was greater than 100 mg / L. However, the granular sludge obtained in Example 1 of the present invention was not damaged, and after shaking for 24 hours, the turbidity of the supernatant was still less than 50 mg / L. It shows that the mechanical strength of the granular sludge obtained by the method of the present invention is relatively high.

[0027] (2) Nitrogen removal effect

[0028] A sewage ...

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Abstract

The invention relates to a rapid culture method for denitrification granular sludge. The rapid culture method comprises the following steps: (1) inoculating an aerobic reactor with sludge to allow MLSS to be 3000 to 4000 mg / L after inoculation, and carrying out enrichment culture on aerobic sludge with low-ammonia-nitrogen high-COD nutrient solution under the condition of alternative high and lowdissolved oxygen contents; (2) taking out a half of the sludge having undergone enrichment culture in the step (1), carrying out culture on denitrification granular sludge under the condition of a DOvalue of less than 1.0 mg / L, and adding CaO with a concentration of 5 to 10 mg / L into a system; (3) mixing the denitrification granular sludge obtained in the step (2) with the other half of the sludge having undergone enrichment culture in the step (1), carrying out reinforced culture on the denitrification granular sludge by using a high-ammonia-nitrogen low-COD nutrient solution under the condition of a DO value of greater than 5 mg / L, and enriching nitrosobacteria; and (4) subjecting the granular sludge obtained in the step (3) to optimized culture under the condition of a DO value of 1 to2 mg / L by using the same culture manner and the same nutrient solution as adopted in the step (3). The denitrification granular sludge cultured by using the method has high activity, stable performance and good total nitrogen removal effect and realizes stable operation of SND indeed.

Description

technical field [0001] The invention belongs to the technical field of wastewater biological treatment, and in particular relates to a rapid cultivation method capable of realizing synchronous nitrification, denitrification and denitrification granular sludge. Background technique [0002] In the 1990s, with the treatment of organic industrial wastewater, granular sludge technology appeared in some anaerobic reactors. Granular sludge is a granular microbial aggregate with good settling performance formed by the aggregation and growth of a large number of bacteria, which is the key and prerequisite for the efficient operation of the reactor. Compared with flocculent activated sludge, granular sludge has the advantages of outstanding settling performance, high sludge density, high physical strength, large biomass and strong load bearing capacity. [0003] The relatively mature granular sludge technology is mainly used in the treatment of wastewater containing organic pollutan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/30C02F11/02
CPCC02F3/302C02F11/02
Inventor 高会杰孙丹凤郭志华赵胜楠陈明翔李宝忠
Owner CHINA PETROLEUM & CHEM CORP