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Anti-hepatitis B virus composition and medicament and use thereof

A composition, compound technology, applied in the direction of antiviral agents, drug combinations, biochemical equipment and methods, etc., can solve problems such as limited treatment options, and achieve the effect of significant benefits and properties

Active Publication Date: 2021-10-15
ARCTURUS THERAPEUTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liver cancer progresses rapidly with limited treatment options

Method used

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  • Anti-hepatitis B virus composition and medicament and use thereof
  • Anti-hepatitis B virus composition and medicament and use thereof
  • Anti-hepatitis B virus composition and medicament and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0405] Example 1: Determination of luciferase reporter.

[0406] PSICHECK TM 2 carrier (Promega, Madison, Wi) constructs luciferase-based reporter plasmid. The report sub-P (1-20) is produced using an oligonucleotide containing a sequence of the ECO Ri digestive site from the position 1 to 2500, which is cloned to psicheck. TM 2 In the doubts of the destinillary of the sacred luciferase gene started by the SV40, the psicheck TM 2 The sea-kidney luciferase gene is expressed under the regulation of artificial 3'UTR sequences. The sea-kidney luciferase activity is then used as an indicator of the effect of artificial 3'UTR on the stability and translation efficiency of transcript. Psicheck TM The -2 vector also contains a form-form expression of firefly luciferase gene, which is used as an internal control to normalize the transfection efficiency.

[0407] The day before transfection, a total of 5,000 HepB3 cells (American Type Culture Collection (AmericanType Culture Collection)) pl...

Embodiment 2

[0409] Example 2: The HBV inhibitory effect of UNA oligomers was observed using PSICHECK2. At 1 nm concentration last for 6 days, it was determined that the percentage of the target gum of each UNA oligomeric compound having a reference position 1578 was 77% to 97%. Thus, all UNA oligomeric compounds having reference positions 1578 in Table 19 are operable to make target expression silencing.

Embodiment 3

[0410] Example 3: The HBV inhibitory effect of UNA oligomers was measured by PSICHECK2. At 1 nm concentration last for 6 days, it was determined that the target gum of each UNA oligomeric compound having reference position 1777 was 77% to 92%. Thus, all UNA oligomeric compounds having reference position 1777 in Table 19 are operable to make target expression silencing.

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Abstract

The present invention encompasses compounds and compositions for use in methods of medical treatment, generally for inhibiting hepatitis B virus in a subject. The compound has a first chain and a second chain, each of the chains has a length of 19-29 monomers, the monomers include UNA monomers and nucleic acid monomers, and the compound targets HBV genome sequence.

Description

Technical field [0001] The present invention relates to a biological drug and therapeutic sector consisting of oligomers for gene silencing. More specifically, the present invention relates to structures, compositions and methods of therapeutic oligomers for hepatitis B viruses. [0002] Sequence list [0003] The present application includes a sequence list in the form of electronics submitted by ASCII file named Arc1410WO_SL.TXT. Background technique [0004] Hepatitis B is liver disease caused by hepatitis B virus (HBV) infection. Its severity can be from a few weeks of slight illness to severe lifetime diseases. Hepatitis B can be acute or chronic. Acute hepatitis B virus infection is a short-term disease that can lead to chronic infections. Chronic hepatitis B virus infections can lead to long-term health problems (such as cirrhosis, liver cancer and death). [0005] Hepatitis B is typically propagated by contacting the transfer body fluid with the infected person or by shar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K31/713A61P31/20
CPCC12N2310/14C12N2310/323C12N2310/344C12N15/1131A61P1/16A61P31/20
Inventor P·林姆冯立川洁C·以扫帕德马纳巴·契吾库拉
Owner ARCTURUS THERAPEUTICS