Pyridin-3-yl acetic acid derivatives as inhibitors of human immunodeficiency virus replication
A technology of pyrrolidinyl and tetrahydropyranyl, which is applied to new HIV inhibitors, uses these compounds in the treatment of HIV infection, and prepares the fields of compounds described below, which can solve problems such as viral drug resistance
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Embodiment 1
[0340]
[0341] (S)-2-(5-(4-(Benzylcarbamoyl)phenyl)-4-(2-azabicyclo[2.2.1]hept-2-yl)-2,6-dimethyl Pyridin-3-yl)-2-(tert-butoxy)acetic acid: Add 0.13 mL of 1M sodium hydroxide (5.14 mg, 0.13 mmol) to (S)-2-(5-(4-(benzylcarbamoyl)phenyl)-4-(2-azabicyclo[2.2. 1] Hept-2-yl)-2,6-dimethylpyridin-3-yl)-2-(tert-butoxy) isopropyl acetate (30 mg, 0.05 mmol) in ethanol (2.5 mL) solution and stirred at 90°C for 18 hours. The reaction mixture was neutralized with 1N HCl solution, extracted with EtOAc, and the organic layer was washed with brine and dried (MgSO 4 ). The crude material was purified by preparative HPLC to afford (S)-2-(5-(4-(benzylcarbamoyl)phenyl)-4-(2-azabicyclo[2.2.1]hept-2 -yl)-2,6-dimethylpyridin-3-yl)-2-(tert-butoxy)acetic acid 12.4 mg (45%).
[0342]
[0343]
[0344] 2-(5-Bromo-4-(3,4-dihydroisoquinolin-2(1H)-yl)-2,6-dimethylpyridin-3-yl)-2-oxoacetic acid iso Propyl ester: 1,2,3,4-Tetrahydroisoquinoline, HCl (1.39 g, 8.22 mmol) and DIEA (2.61 mL, ...
Embodiment 2
[0357]
[0358] (S)-2-(tert-butoxy)-2-(4-(3,4-dihydroisoquinolin-2(1H)-yl)-5-(4-(4-fluorophenethoxy )phenyl)- 2,6-Dimethylpyridin-3-yl)acetic acid: Add 0.10 mL of 1M sodium hydroxide (4.0 mg, 0.10 mmol) to (S)-2-(tert-butoxy)-2-(4-(3,4-dihydroisoquinoline-2(1H)- yl)-5-(4-(4-fluorophenethoxy)phenyl)-2,6-dimethylpyridin-3-yl) isopropyl acetate (25 mg, 0.04 mmol) in ethanol (1.5 mL ) and stirred at 90°C for 18 hours. An additional 0.1 mL of sodium hydroxide solution was added and the reaction continued for 18 hours. The reaction mixture was neutralized with 1N HCl solution, extracted with EtOAc, and the organic layer was washed with brine and dried (MgSO 4 ). The crude material was purified by preparative HPLC to give (S)-2-(tert-butoxy)-2-(4-(3,4-dihydroisoquinolin-2(1H)-yl)-5- (4-(4-Fluorophenethoxy)phenyl)-2,6-dimethylpyridin-3-yl)acetic acid 8.5 mg (37%).
[0359]
[0360]
[0361] 2-(5-bromo-4-(hexahydrocyclopenta[c]pyrrol-2(1H)-yl)-2,6-dimethylpyridin-3-...
Embodiment 3
[0375]
[0376] (S)-2-(5-(4-(Benzylcarbamoyl)phenyl)-4-(2-azabicyclo[2.2.1]hept-2-yl)-2,6-dimethyl Pyridin-3-yl)-2-(tert-butoxy)acetic acid: Add 0.40 mL of 1M sodium hydroxide (15.9 mg, 0.40 mmol) to (2S)-2-(tert-butoxy)-2-(5-(4-(4-fluorophenethoxy)phenyl)- 4-(Hexahydrocyclopenta[c]pyrrol-2(1H)-yl)-2,6-dimethylpyridin-3-yl)isopropyl acetate (60 mg, 0.10 mmol) in ethanol ( 2 mL) and stirred at 90°C for 18 hours. The reaction mixture was neutralized with 1N HCl solution, extracted with EtOAc, and the organic layer was washed with brine and dried (MgSO 4 ). The crude material was purified by prep-HPLC to obtain (S)-2-(5-(4-(benzylcarbamoyl)phenyl)-4-(2-azabicyclo[2.2.1]heptane- 2-yl)-2,6-dimethylpyridin-3-yl)-2-(tert-butoxy)acetic acid 21.5 mg (38%).
[0377]
[0378]
[0379] (2S)-2-(5-(4-(Benzylcarbamoyl)phenyl)-4-(hexahydrocyclopenta[c]pyrrole-2(1H)- Base)-2,6-dimethylpyridin-3-yl)-2-(tert-butoxy)acetate isopropyl ester: Pd(Ph 3 P) 4 (54 mg, 0.047 mmol) ...
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