A strain of Streptococcus thermophilus with high exopolysaccharide production, storage and culture method and method for preparing fermented milk using the Streptococcus thermophilus

Abstract

The invention discloses a Streptococcus thermophilus with high exopolysaccharide production, and its preservation number is CGMCC No.14809; the application also discloses a method for preparing fermented milk using the Streptococcus thermophilus with high exopolysaccharide production, including the following Steps: (1) activate the Streptococcus thermophilus with high exopolysaccharide production using M17 slant medium; (2) add the Streptococcus thermophilus with high exopolysaccharide production after activation in step (1) into the liquid medium for anaerobic Oxygen cultivation; (3) inoculating the liquid seed cultivated in step (2) with the milk product to be fermented according to the volume ratio of 5% to 10%, and then anaerobically fermenting to obtain fermented milk rich in exopolysaccharide. The extracellular polysaccharide-producing Streptococcus thermophilus of the present invention can reduce or completely replace the use of food additives in the fermentation process, so that there is no need to add stabilizers, and at the same time, the exopolysaccharide-producing Streptococcus thermophilus also has the ability to improve product texture The effect of improving the water retention and smoothness of the product.

Description

technical field [0001] The invention belongs to the technical field of microbes and microbial fermentation, in particular to a Streptococcus thermophilus strain with high exopolysaccharide production, a preservation and cultivation method and a method for preparing fermented milk by using the Streptococcus thermophilus. Background technique [0002] Lactic acid bacteria exopolysaccharide (EPS) is a type of carbohydrate compound secreted by lactic acid bacteria outside the cell wall during growth and metabolism. Some of them attach to the microbial cell wall to form capsules, called capsular polysaccharides; some enter the culture medium to form mucus. Known as mucus polysaccharides, it is the product of microbial adaptation to the environment. Lactic acid bacteria EPS has significant physiological functions: (1) anti-cancer and anti-tumor effects; (2) promote the adsorption of probiotics on the intestinal mucosa. (3) It has a promoting effect on the regulation of the immune...

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Problems solved by technology

[0004] In order to solve the defect in the prior art that stabilizers, such as starch, pectin, agar, etc. need to be added during the fermentation process of yogurt to reduce whey precipitation during the shelf life of the product, the present invention provides a strain of thermophilic chain with high production of exopolysaccharide Coccus, preservation and culture method and method for preparing fermented milk by using the Streptococcus thermophilus, the purpose achieved is that the strain can grow rapidly in milk and produce a higher yield of exopolysaccharide, which has great application value

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