A method for preparing purified polypeptide or peg-modified gold nanoparticles without centrifugation
A gold nanoparticle, nanoparticle technology, applied in nanotechnology, nanotechnology, nanotechnology and other directions for materials and surface science, can solve the problem of time-consuming and laborious separation of gold nanoparticles
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Embodiment 1
[0017] 13nm gold nanoparticles preparation method:
[0018] 1) Get 100mL milli-Q water in the three-necked bottle, heat the oil bath with magnetic stirring and reflux, add 2mL 1wt% HAuCl and 4mL sodium citrate solution (the amount of the substance is 30mmol) to it successively after boiling, the solution color is from Pale yellow turns to light gray, blue, and eventually wine red. Continue heating for 20min, stir and cool to room temperature to obtain 13nm gold nanoparticles, see TEM image figure 1 .
[0019] 2) Add 100μL 1mg / mL CALNN to 1mL sodium citrate-protected 13nm gold particles with an absorbance (optical density, OD) of 1, mix well, and place at room temperature for more than 2 hours. The surface charge of CALNN-modified gold nanoparticles is -38.6mV .
[0020] 3) Add 0.1M HCl until the color of the solution turns blue-gray, let it stand still, wait for the gold nanoparticles to precipitate, and carefully remove the supernatant.
[0021] 4) Add 10 μL of 10 mM phos...
Embodiment 2
[0025] Preparation method of 8nm gold particles: add 375 μL 4wt% HAuCl4 and 500 μL 0.2M potassium carbonate in 100 mL milli-Q water successively, stir evenly in an ice-water bath, add 5 mL of newly prepared sodium borohydride (0.5 mg / mL) dropwise to the above In the mixed solution, the color of the solution gradually changed from blue-purple to bright red, and the stirring was continued for 30 minutes.
[0026] Add 100 μL of 1 mg / mL GSH to 1 mL of potassium carbonate-protected 8 nm gold particles with an absorbance of 1, mix well, and place at room temperature for more than 2 hours. The average surface charge of the GSH-modified gold nanoparticles is -28.2 mV.
[0027] Add 0.1M HCl until the color of the solution turns blue-gray, let stand until the gold nanoparticles precipitate, and carefully remove the supernatant.
[0028] Add 10 μL of 10 mM phosphate buffer (pH 7.4) to the precipitate, it can be observed that the precipitate turns from gray to red, and add water to 500 μL...
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