Nucleic acid purification reagent based on magnetic bead method

Abstract

The invention relates to a nucleic acid purification reagent based on a magnetic bead method and a kit. The nucleic acid purification reagent is prepared from a water solution containing the followingsubstances in percentage by mass: 1 to 5 percent of carboxyl-modified magnetic beads, 11.78 to 23.56 percent of sodium chloride, 10 to 20 percent of polyethylene glycol, 0.01 to 0.05 percent of N-sodium lauroyl sarcosine, 0.16 to 0.79 percent of Tris-HCl, 0.5 to 2 percent of EDTA and 0.5 to 2 percent of tween-20. A nucleic acid purification recovery kit comprises the nucleic acid purification reagent, a washing buffer solution and an elution buffer solution. The nucleic acid purification reagent and the kit have the advantages that the nucleic acid recovery efficiency is higher than the recovery rate of the existing magnetic bead type reagent and kit; the recovery loss on trace samples is lower; the recovery efficiency on nucleic acid fragments being about 100bp or smaller nucleic acid fragments is better.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a nucleic acid purification reagent based on a magnetic bead method. Background technique [0002] At present, the purification of nucleic acid is inseparable from all fields of biomedicine. Many reactions, such as next-generation sequencing and TA cloning, require the purification and recovery of nucleic acid after enzymatic reactions. There are many ways to separate and purify nucleic acid: The concentrated salt method that dissolves different characteristics of RNA and RNA in a salt solution to separate the two, the organic solvent extraction method that uses organic solvents to denature proteins while inhibiting nuclease degradation, and the density of nucleic acids that are separated by using the principle of different densities of different contents Gradient centrifugation, as well as the adsorption material binding method that uses different adsorption materials (such as s...

AI Technical Summary

Problems solved by technology

If you encounter problems such as many steps in the operating system, difficult sampling of rare samples to be processed, and low initial volume of samples to be processed, how to efficiently perform nucleic acid purification and recovery in the experimental process plays a vital role in the results of the experimental operation, and liquid biopsy Many fragments of cfDNA in China are about 100bp or less in size, and the recovery rate of such fragments is very low for existing nucleic acid purification reagents based on magnetic beads

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