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Rapid Biomonitoring Method of Chrysanthemum Sanmao toxins in Water

A technology of golden algae and toxins, applied in the field of breeding, can solve the problems of lack of physical and chemical analysis methods, difficult observation, and economic losses of farmers

Active Publication Date: 2021-05-14
合肥九州龙腾科技成果转化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chrysophylla is afraid of sunlight, so there are much fewer Chrysophytes in the surface water than in waters below 0.4 meters, and it is difficult to observe it by routine water quality sampling
Fish diseases caused by chrysotoxins have occurred in all major aquaculture areas of the country, causing varying degrees of economic losses to farmers
The reason is mainly that the current farmers do not know enough about Chrysanthemum trichamota, so there is little research on it in the academic circles. There is no complete set of physical and chemical analysis methods for chrysotoxins. In production, it mainly relies on morphological microscope inspection, lacking reliable physical and chemical analysis methods. Analytical means, resulting in the inability to detect the toxicity level of Chrysanthemum trichamota, so that effective measures cannot be taken in time

Method used

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  • Rapid Biomonitoring Method of Chrysanthemum Sanmao toxins in Water
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  • Rapid Biomonitoring Method of Chrysanthemum Sanmao toxins in Water

Examples

Experimental program
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Effect test

Embodiment 1

[0045] On the morning of November 20, 2017, in a bream breeding pond in Yancheng, Jiangsu Province (average body length 6cm), it was found that there were bream swimming under the water surface on the edge of the pond. ; On November 21, several breams were found to be dead; 4 breams that were on the verge of death were randomly selected, and the base of the surface fins was observed to be congested, and the color of the rear of the fish became lighter; There were no parasites on the gill filaments, and the gill filaments were normal (slightly rotten); the fish body was dissected, the intestinal tract was devoid of food, and there were no obvious lesions; the color of the pond water was brown, the pH value was 9.0, and almost no ammonia nitrogen and nitrite were detected ( close to 0). Based on the analysis of the above situation, there is a problem with the water quality. Combined with the normal results of the water quality test, the possibility of algae poisoning in the wate...

Embodiment 2

[0050]In a farm in Taizhou, Jiangsu Province, grass goldfish (average body length 10cm) were raised in winter in the outer earthen pond, and a large number of fish died. Water quality testing: pH dropped significantly, ammonia nitrogen and nitrite were normal, but the water hardness was 1000mg / L, which was high. Detection of algae in aquaculture water: there are a large number of golden algae, cryptoalgae and some zooplankton. Compared with Table 1, the following experimental design scheme was adopted and relevant results were recorded. The results are shown in Table 3.

[0051] The test result of table 3 embodiment 1

[0052]

[0053] Looking up Table 1, it is found that the toxicity of golden algae in cultured water is greater than or equal to 25ITU / mL.

Embodiment 3

[0055] On April 13, 2016, in Taizhou, Jiangsu Province, a breeding base of scallop seedlings (average body length 3cm), one of the 6 mu ponds was full of yellow water, the transparency was less than 20cm, and there were a lot of golden algae in the microscopic examination. Compared with Table 1, the following experimental design scheme was adopted and relevant results were recorded. The results are shown in Table 4.

[0056]

[0057] According to Table 1, the toxicity of golden algae in aquaculture water is equal to 1ITU / mL, and continuous monitoring is recommended.

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Abstract

The invention discloses a rapid biological monitoring method for chrysotoxin in aquaculture water, comprising the following steps: (1) preparing three groups of test waters of equal volume, wherein the test water of the first group is all aquaculture water; The test water in the test is a mixed solution composed of aquaculture water and auxiliary test solution; the test water in the third group is a mixed solution composed of aquaculture water, double distilled water and auxiliary test solution; (2) add the amount of Equal fish; (3) record the number of deaths of fish in each group after 2 hours, and judge the toxicity of chrysotoxin in the culture water according to the death situation of fish; 1M Tris buffer solution, the concentration of the 3,3'-diaminodipropylamine is 0.14-0.16M. The monitoring method has the advantages of simple operation, strong practicability, fast detection speed and high accuracy.

Description

technical field [0001] The invention relates to the field of breeding technology, in particular to a rapid biological monitoring method for chrysotoxin in water bodies. Background technique [0002] Chrysophorus trichamota is a kind of euryhaline algae. When it multiplies in large numbers, it secretes cytotoxins into the water, which in turn poisons fish and aquatic animals to death. Chrysophytes occur in all seasons, mainly in spring, autumn, and winter. Because the water temperature is low at this time, other algae are affected by the low temperature, reproduce slowly, and the number is small. The low-temperature-resistant Chrysophylla becomes the dominant species and causes harm. Chlorella trichamota is afraid of sunlight, so there are much fewer Chrysanthemum trichamota in the surface water than in waters below 0.4 meters, and it is difficult to observe it in conventional water quality sampling. The harm process of Chrysophyllum trichamota can be divided into three stag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/18
CPCG01N33/186
Inventor 封琦王庆韩晓磊周子健曹宇王建国王权
Owner 合肥九州龙腾科技成果转化有限公司