Application of long-chain non-coding RNA and composition thereof to diagnosis and treatment of cancer of biliary duct
A long-chain non-coding and composition technology, applied to long-chain non-coding RNA, the application in the preparation and diagnosis of cholangiocarcinoma and target drug therapy, and the application field in the preparation of reagents for diagnosing cholangiocarcinoma, which can solve the poor prognosis of cholangiocarcinoma patients , malignant progression of cholangiocarcinoma, etc.
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[0070] The present invention is further illustrated below with examples, but the present invention is not limited thereto. For the experimental methods without specific conditions indicated in the following examples, the conventional conditions or the conditions suggested by the manufacturer are usually followed.
[0071] 1. Bioinformatics screening and identification of high expression of PVT1 in cholangiocarcinoma
[0072] (1) The inclusion criteria entered the GEO database ( http: / / www.ncbi.nlm.nih.gov / gds), Query "cholangiocarcinoma" and "cancer of biliary duct", limit the filter conditions "Entry type: Series", "Organism: Homo sapiens", "Study type: Expression profiling by array" and "Attribute name: tissue", get information about cholangiocarcinoma According to the results of microarray research query, select research projects that meet the following requirements: cholangiocarcinoma, case-control (or cancer-adjacent), mainstream microarray platforms (Affy, Agilent, and...
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