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Method for cultivating rodgersia aesculifolia plants

A breeding method, the technology of C. chinensis, which is applied in the field of rapid cultivation of C. chinensis medicinal plants, can solve problems such as the inability to maintain good genetic traits stably, achieve good market development value and economic benefits, maintain genetic traits, and increase reproduction speed. Effect

Inactive Publication Date: 2018-07-06
ANKANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the above-mentioned problems existing in the planting of the existing medicinal plant C. chinensis, the main purpose of the present invention is to provide a technique for the rapid propagation of plant tissue of the medicinal plant C. spp. The problem that the traits cannot be maintained stably

Method used

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Embodiment Construction

[0019] The present invention will be further described below with reference to specific examples.

[0020] A method for cultivating Cauliflower plants of the present invention comprises the following steps:

[0021] (1) Cut the young leaves from the adult Cauliflower as explants, put them into the callus induction medium to induce the formation of callus; the callus induction medium is MS+6-BA, NAA The mixed composition is composed of MS+6-BA1.0mg / L+NAA 0.1mg / L.

[0022] (2) Take out the granular callus and put it into the bud induction differentiation medium to induce the callus to form adventitious buds; the bud induction differentiation medium is mixed with MS+6-BA and NAA, consisting of MS+6 -BA 2.0mg / L+NAA 0.1mg / L mixed composition.

[0023] (3) Take out the adventitious buds formed and put them into the adventitious bud proliferation medium to form a large number of clustered buds; the adventitious bud proliferation medium is a mixture of MS+6-BA and NAA, consisting of...

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Abstract

The invention discloses a method for cultivating and rapidly propagating tissues of medicinal rodgersia aesculifolia plants. The method comprises the following steps: (1) cutting young leaves from anadult rodgersia aesculifolia plant as an explant, sterilizing by mercury and alcohol, and then placing into a callus-inducing culture medium for inducing to form calluses; (2) taking out granular calluses, and placing into a bud-inducing differentiation culture medium for inducing differentiation of callus cells to form adventitious buds; (3) placing the formed adventitious buds into a bud proliferating culture medium to form a large number of cluster buds; and (4) taking out the cluster buds, and placing into an induction rooting culture medium for inducing rooting to form regenerated seedlings. The method provided by the invention can greatly improve a propagating speed of the medicinal rodgersia aesculifolia plants, and can well maintain original maternal characters, and has a high survival rate and good economic effects.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for rapidly cultivating medicinal plants of C. chinensis. Background technique [0002] Aescin is a perennial herbaceous plant of the genus Aescinaceae in the saxifrage family. As a traditional folk herbal medicine in my country, it has a long history of application and good clinical efficacy. The main components of Aescin are petogenin, catechin, gallic acid, arbutin, etc., all of which have strong physiological and pharmacological activities. Its special effects in clearing heat and removing dampness, stopping diarrhea, stopping bleeding, treating irregular menstruation and rheumatoid arthritis have been studied and concerned by many scholars at home and abroad. There have been few large-scale artificial cultivations of Aesculus aesculus for a long time, and most of them are in the wild state. During the natural growth and long-term evolution process, due to the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王健
Owner ANKANG UNIV
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