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Preparation method and application of traditional Chinese medicine composition leavening alcohol extraction ingredient

A technology combining fermentation and traditional Chinese medicine, applied in the field of traditional Chinese medicine, to achieve the effects of strong antiviral activity and simple and reasonable preparation process

Inactive Publication Date: 2018-07-06
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As of the end of April 2012, there are 63 varieties of antiviral drugs approved for clinical use in the world, and these drugs can only prevent and treat diseases caused by a few viruses

Method used

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  • Preparation method and application of traditional Chinese medicine composition leavening alcohol extraction ingredient
  • Preparation method and application of traditional Chinese medicine composition leavening alcohol extraction ingredient
  • Preparation method and application of traditional Chinese medicine composition leavening alcohol extraction ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Preparation process of traditional Chinese medicine combination fermented product.

[0015] The traditional Chinese medicine combination fermented product is prepared by the following method.

[0016] (1) An appropriate amount of Beauveria bassiana was aseptically inoculated on sterilized PDA medium, cultured for 7 days, and the second-generation cultured strain was diluted with sterile distilled water to 1×10 8 spores / mL.

[0017] (2) Precisely weigh 10.000g of sterilized scorpion powder, Atractylodes macrocephala powder and grub powder in a petri dish, and add the dilution of the above spores. Ferment for 7 days in a constant temperature and humidity incubator, and stop the fermentation after the mycelium is overgrown.

Embodiment 2

[0018] Example 2 The preparation process of alcoholic extraction components of traditional Chinese medicine combination fermented product.

[0019] The following method is used to prepare the alcoholic extract components of traditional Chinese medicine combination fermented products.

[0020] (1) Take out the above-mentioned mixed fermented product and put it into a freeze dryer for freeze-drying.

[0021] (2) Accurately weigh 2.000 g of fermented Scorpion, Atractylodes macrocephala, and grub powder, and extract them twice for 2.5 hours in an 80°C water bath according to the ratio of medicine to 50% ethanol (1:40).

[0022] (3) Suction filter the extract, and the liquid obtained by suction filtration is the alcohol extract, which is evaporated to dryness in a 45°C water bath to obtain 50% alcohol extract.

Embodiment 3

[0023] Example 3 Biological test.

[0024] The beneficial effects of the present invention will be further illustrated by experiments below. The alcohol extract of fermented traditional Chinese medicine composition of the present invention has the following beneficial effects, but it should not be understood that the present invention only has the following beneficial effects.

[0025] Experiment 1. Toxic effect of alcohol extract components of traditional Chinese medicine combination fermentation products on MA104 cells

[0026] (1) Take the MA104 cell seeds from the ultra-low temperature refrigerator, and quickly put them into the water preheated to 37°C. After the frozen storage solution melts, centrifuge at 1000 r / min for 3 minutes to collect the cells, and then add 1640 cells containing 10% fetal bovine serum. fresh medium at 37°C, 5% CO 2 cultured in an incubator.

[0027] (2) Digest MA104 cells in the logarithmic phase of growth, and use 1×10 5 seeded in 96-well pla...

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PUM

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Abstract

The invention discloses a preparation technology of a traditional Chinese medicine composition leavening and a traditional Chinese medicine composition leavening alcohol extraction ingredient, and application of the alcohol extraction ingredient. The mixture of beauveria bassiana fermented scorpio, rhizoma atractylodis macrocephalae and grub is adopted, and then the alcohol extraction ingredient is obtained after freeze-drying and 50% ethyl alcohol extraction. The preparation technology is simple, and the inhibiting effect of the obtained leavening alcohol extraction ingredient for viruses such as RSV, EV71, COX-B2 and HSV is superior to that of ribavirin. The alcohol extraction ingredient can be prepared into the dosage forms such as capsules, tablets, a lung suction powder inhalation anda nose suction powder inhalation for antiviral therapy.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to an antiviral traditional Chinese medicine composition, and also relates to a preparation method and application of the composition. Background technique [0002] Virus infection can cause various diseases and seriously endanger human health and life. According to incomplete statistics, about 60% of epidemic infectious diseases are caused by virus infection. At present, there are more than 1,200 kinds of viruses that can cause human diseases and have been confirmed by the International Virus Taxonomy Committee. As of the end of April 2012, there are 63 varieties of antiviral drugs approved for clinical use in the world, and these drugs can only prevent and treat diseases caused by a few viruses. Therefore, the task of developing new antiviral drugs is urgent. In this study, fermentation technology was used to process the mixture of traditional Chinese medic...

Claims

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Application Information

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IPC IPC(8): A61K36/284A61P31/14A61P31/22A61K35/646A61K35/64
CPCA61K35/646A61K35/63A61K36/284A61K2236/19A61K2236/333A61K2236/39A61K2236/51A61K2300/00
Inventor 高世杰王俊香张珊珊王集会张瑞
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE