Extraction method and application of silverfish polysaccharide
An extraction method and technology of silverfish, which are applied in the field of extracting polysaccharides from silverfish, can solve the problems that the report of using silverfish has not yet been found, and achieve the effects of enhancing solubility, saving costs, and improving immune function
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Embodiment 1
[0026] A method for extracting polysaccharides from silverfish, comprising the steps of:
[0027] (1) Get fresh silverfish, wash and dry, then pulverize it, and cross a 50-mesh sieve to obtain silverfish meal;
[0028] (2) Add the fish meal of step (1) into 65°C water, add NaOH of 1mol / L concentration to adjust the concentration of dilute lye to be 0.02mol / L, then add NaCl to adjust the sodium ion concentration to be 0.05mol / L, and extract 2 hour, filter and collect the filtrate;
[0029] (3) After adding the dehydrated alcohol of 4 volume ratios to the filtrate of step (2), centrifugation, the centrifugal speed is 4000r / min, and the precipitate is collected;
[0030] (4) Rinse the precipitate in step (3) with absolute ethanol, and air-dry to obtain silverfish polysaccharide.
Embodiment 2
[0032] A method for extracting polysaccharides from silverfish, comprising the steps of:
[0033] (1) Get fresh silverfish, wash and dry, then pulverize it, and cross a 40-mesh sieve to obtain silverfish meal;
[0034] (2) Add the silver fish meal of step (1) into 50°C water, add NaOH of 1mol / L concentration to adjust the concentration of dilute lye to 0.01mol / L, then add NaCl to adjust the sodium ion concentration to 0.05mol / L, and extract 1 hour, filter and collect the filtrate;
[0035] (3) After adding the dehydrated alcohol of 3 volume ratios to the filtrate of step (2), centrifuge, the centrifugal speed is 3000r / min, and collect the precipitate;
[0036] (4) Rinse the precipitate in step (3) with absolute ethanol, and air-dry to obtain silverfish polysaccharide.
Embodiment 3
[0038] A method for extracting polysaccharides from silverfish, comprising the steps of:
[0039] (1) Get fresh silverfish, wash and dry, then pulverize it, and cross a 60-mesh sieve to obtain silverfish meal;
[0040] (2) Add the silverfish powder of step (1) into 80°C water, add NaOH of 1mol / L concentration to adjust the dilute lye concentration to be 0.03mol / L, then add NaCl to adjust the sodium ion concentration to be 0.05mol / L, and extract 3 hour, filter and collect the filtrate;
[0041] (3) After adding the dehydrated alcohol of 5 volume ratios to the filtrate of step (2), centrifugation, the centrifugal speed is 5000r / min, and the precipitate is collected;
[0042] (4) Rinse the precipitate in step (3) with absolute ethanol, and air-dry to obtain silverfish polysaccharide.
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