A method for the joint extraction of essential oil, pectin, hesperidin, synephrine and limonin from citrus
A joint extraction and limonin technology, applied in the field of hesperidin, pectin, synephrine and limonin, combined extraction of essential oils, can solve the problem of reduced economic value of citrus peel processing and utilization, can not achieve joint extraction, citrus peel Problems such as low utilization rate can achieve the effect of reducing enzymatic hydrolysis time and enzyme consumption, saving time and labor costs, and making the production process safe and easy to operate.
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Embodiment 1
[0041] see figure 1 , a method for jointly extracting essential oil, pectin, hesperidin, synephrine and limonin from citrus, comprising the steps of:
[0042] (1) Take fresh and clean citrus, inactivate plant enzymes at 100 °C for 8 min, pierce the epidermis with a needle-punched dermabrasion machine, rinse the citrus surface with water spray, and collect the oil-water mixture, skin residue and dermabrasion citrus respectively.
[0043] (2) Enzymatically hydrolyze the pomace and peeled citrus with cellulase, specifically: add 11.5% ammonium sulfate to the mixture composed of pomace and dehulled citrus according to the ratio of solid to liquid (w / v) of 1:8 and 0.4% cellulase, shaken in a water bath at 45°C for 45 minutes; then heated at 85°C for 5 minutes, and ultrasonically extracted for 90 minutes to obtain enzymatic hydrolysis products. Squeeze and filter the enzymatic hydrolysis product with a screw press, and collect the squeeze liquid and skin dregs respectively.
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Embodiment 2
[0076] Investigate the influence of different cation exchange resins on the extraction effect of synephrine:
[0077] Weigh 30 mL of processed cation exchange resins (732, D72, Dowex 50(H+), DL08, DL10, D006) of different types, wet-pack the column (300 mm×15 mm), and adjust the volume flow rate to 2.5 BV / h , each adding the aqueous phase collected in step (3) of Example 1, collecting the effluent, eluting with ethyl acetate, and collecting the eluate; measuring the mass concentration of synephrine in the effluent and each eluate, and calculating Adsorption capacity and desorption rate, Table 1 shows the results of adsorption capacity and analytical capacity of different cation exchange resins.
[0078] Adsorption rate (%) = (C 0 -C 1 ) / C 0 ×100
[0079] Desorption rate (%) = (C 2 / ) C 0 -C 1 ))×100
[0080] In the formula: C 0 is the solution concentration of the target component in the initial sample, mg / mL;
[0081] C 1 is the concentration of the target componen...
Embodiment 3
[0087] Investigate the influence of different macroporous resins on the extraction effect of limonin:
[0088] Weigh 2.0 g each of the 7 kinds of macroporous resins that have been processed, add 30 mL of the supernatant obtained in step (6) of Example 1, and let it stand at room temperature for sufficient adsorption to reach saturation (24 h) and filter to measure the adsorption The content of limonin in the sample solution before and after and the content of limonin in the eluate after elution with 70% ethanol, Table 2 shows the results of the adsorption capacity and analytical capacity of different macroporous resins.
[0089] Calculate the adsorption rate and desorption rate of limonin by the macroporous resin according to the following formula.
[0090] Adsorption rate (%) = (C 0 -C1 ) / C 0 ×100
[0091] Desorption rate (%) = (C 2 / ) C 0 -C 1 ))×100
[0092] In the formula: C 0 is the solution concentration of the target component in the initial sample, mg / mL;
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